HLA-B7 Antibody (BB7.1)recommended for detection of HLA-B7 of human origin by FCM

HLA-B7 Antibody (BB7.1): sc-53304

HLA-B7 Antibody (BB7.1) is rated 2.5 out of 5 by 2.
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Datasheets

    • HLA-B7 Antibody (BB7.1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against HLA-B7 of human origin
    • HLA-B7 Antibody (BB7.1) is recommended for detection of HLA-B7 of human origin by FCM
    • HLA-B7 Antibody (BB7.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HLA-B7 (BB7.1): sc-53304.
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Can HLA-B7 (BB7.1): sc-53304 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, HLA-B7 (BB7.1): sc-53304 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:10
Date published: 2018-11-19
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