HLA-B/C Antibody (A-3)recommended for detection of HLA-B and HLA-C of human origin, H2-L and H2-Q of mouse origin, and the corresponding rat homologs by WB, IP, IF and ELISA

HLA-B/C Antibody (A-3): sc-166668

HLA-B/C Antibody (A-3) is rated 5.0 out of 5 by 2.
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Datasheets

    • HLA-B/C Antibody (A-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 126-145 within an internal region of HLA-C of human origin
    • HLA-B/C Antibody (A-3) is recommended for detection of HLA-B and HLA-C of human origin, H2-L and H2-Q of mouse origin, and the corresponding rat homologs by WB, IP, IF and ELISA
    • HLA-B/C Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-166668 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HLA-B/C (A-3): sc-166668.
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What application is the blocking peptide sc-166668 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Good with IHC Works fine with IHC (pH 9, 1:50 with blocking in FCS) on human tonsil and brain (5 µm FFPE).
Date published: 2018-04-12
Rated 5 out of 5 by from Produced nice Western blot data of HLA Produced nice Western blot data of HLA-B/C expression in CCRF-CEM whole cell lysate. -SCBT QC
Date published: 2014-03-23
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