HLA-A/B/C Antibody (LY5.1)recommended for detection of HLA-A/B/C of human origin by WB, IP and IF

HLA-A/B/C Antibody (LY5.1): sc-52810

HLA-A/B/C Antibody (LY5.1) is rated 5.0 out of 5 by 1.
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Datasheets

    • HLA-A/B/C Antibody (LY5.1) is a mouse monoclonal IgG1 (kappa light chain) provided at 100 µg/ml
    • raised against purified HLA-A/B/C of human origin
    • recommended for detection of HLA-A/B/C of human origin by WB, IP and IF
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See MHC class I (W6/32): sc-32235 for HLA-A/B/C antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Does this antibody bind to HLA-E?

Asked by: Olivia135
Thank you for your question. This antibody is specific for HLA-A/B/C of human origin and should not cross-react with HLA-E.
Answered by: Technical Support
Date published: 2019-04-11

I am using HLA-A/B/C (LY5.1): sc-52810 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Clean blot with single band for WB in human HLA Clean blot with single band for WB in human HLA-B transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-06-06
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