HLA-A Antibody (B-11)recommended for detection of a broad range of HLA antigens of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

HLA-A Antibody (B-11): sc-390473

HLA-A Antibody (B-11) is rated 4.6 out of 5 by 5.
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Datasheets

    • HLA-A Antibody (B-11) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 26-45 near the N-terminus of HLA-A of human origin
    • recommended for detection of a broad range of HLA antigens of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • blocking peptide, sc-390473 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See MHC class I (W6/32): sc-32235 for HLA-A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HLA-A (B-11): sc-390473.
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What application is the blocking peptide sc-390473 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and gave a satisfactory signal at dilution 1:10.
Date published: 2018-11-19
Rated 5 out of 5 by from Good with IHC Works fine with IHC (pH 6, 1:50) on human tonsil and brain (5 µm FFPE).
Date published: 2018-04-12
Rated 5 out of 5 by from Great Tested this antibody for IF on PFA fixed HeLa cells (both permeabilized and non-permeabilized), at 1:50-75 dilution. The results were good.
Date published: 2017-05-05
Rated 5 out of 5 by from Produced excellent immunofluorescence membrane Produced excellent immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-18
Rated 5 out of 5 by from Stong band in WB in HL Stong band in WB in HL-60 and CCRF-CEM whole cell lysates. -SCBT QC
Date published: 2015-02-04
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