Anti-HGF alpha Antibody (H-10) recommended for detection of HGFα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-HGFα Antibody (H-10): sc-374422

Anti-HGFα Antibody (H-10) is rated 4.9 out of 5 by 10.
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Datasheets

    • Anti-HGFα Antibody (H-10) is a mouse monoclonal IgG1 κ HGFα antibody, cited in 5 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 3-27 at the N-terminus of HGFα of human origin
    • Anti-HGF alpha Antibody (H-10) is recommended for detection of HGFα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and porcine
    • Anti-HGF alpha Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HGFα (H-10): sc-374422.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HGFα Antibody (H-10) for WB and IHC(P) applications. These reagents are now offered in bundles with HGFα Antibody (H-10) (see ordering information below).
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What application is the blocking peptide sc-374422 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great IHC staining Test sample received very quickly, IHC staining performance is great in murine colon sections
Date published: 2017-12-06
Rated 5 out of 5 by from L02 cell it works well in liver normal cell L02 in WB with 1:500 dilution
Date published: 2017-11-22
Rated 5 out of 5 by from HGFα antibody The HGFα antibody is suitable for a variety of experimental methods, IHC diluted with 1/200, dyeing signal is very strong.
Date published: 2017-10-24
Rated 5 out of 5 by from Strong band Strong band obtained in western blot analysis of HGF alpha expression in human kidney tissue extract.
Date published: 2017-05-28
Rated 5 out of 5 by from clean bands Used this antibody in WB in mouse cells and got clean bands for dilution 1:600.
Date published: 2017-04-15
Rated 5 out of 5 by from Excellent product The HGF alpha Antibody (H-10): sc-374422 works very well for immunohistochemistry with formalin fixed, paraffin-embedded tissue sections, showing staining pattern localized to the cytoplasm.
Date published: 2017-02-02
Rated 4 out of 5 by from easy to detect hgf alpha in tumor cells I use it to detect endogenous HGF alpha expression in a549 lung cancer cells and it works good, although higher concentration is needed.
Date published: 2017-01-24
Rated 5 out of 5 by from reliable Immunoperoxidase staining of paraffin embedded human kidney sections using 1:100 dilution,the work is very good and reliable.
Date published: 2016-12-18
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