Anti-HGF Antibody (3F203) recommended for detection of HGF of human origin by WB

Anti-HGF Antibody (3F203): sc-71244

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Anti-HGF Antibody (3F203) is rated 4.0 out of 5 by 1.
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Datasheets

    • HGF Antibody (3F203) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 100 µg/ml
    • raised against recombinant HGF of human origin
    • recommended for detection of HGF of human origin by WB; non cross-reactive with other tested cytokines using indirect ELISA
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HGF Antibody (3F203). This work is in progress.
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How much sample do you recommend loading on the gel for Western blot detection using HGF (3F203): sc-71244?

Asked by: Professor Griffin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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