HEPPSO sodium salt is a zwitterionic hydrogen buffer for biological research. An efficient separator in protein resolution by IEF. A buffer solution of HEPPSO can be prepared by any of several methods. The free acid can be added to water, then titrated with approximately one-hal mole equivalent of sodium hydroxide or potassium hydroxide to the desired pH. Alternatively, equimolar concentrations of HEPPSO and of HEPPSO sodium salt can be mixed in approximately equal volumes and back-titrate with either solution to the appropriate pH. The sodium salt can be titrated with HCl to yield a half-equivalent of sodium chloride; however, the addition of the ionic strength will change the osmolality of the solution.
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