HEPES Sodium saltCAS: 75277-39-3
MF: C8H17N2NaO4S
MW: 260.3
A zwitterionic all-purpose buffer.

HEPES Sodium salt (CAS 75277-39-3)

HEPES Sodium salt | CAS 75277-39-3 is rated 5.0 out of 5 by 1.
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Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
Application: A zwitterionic all-purpose buffer
CAS Number: 75277-39-3
Purity: ≥99%
Molecular Weight: 260.3
Molecular Formula: C8H17N2NaO4S
* Refer to Certificate of Analysis for lot specific data (including water content).
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HEPES Sodium salt (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt) may be used in the preparation of HEPES buffer, which has been described as one of the best all-purpose buffers available for use in biological research. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5.


References

1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Medzon, E.L. and Gedies, A. 1971. Can. J. Microbiol. 17: 651-653. PMID: 5104240
3. Good, N.E. and Izawa, S. 1972. Meth. Enzymol. 24: 53-68. PMID: 4206745
4. Himmel, H.M., et al. 1987. J. Clin. Chem. Clin. Biochem. 25: 909-913. PMID: 3127532
5. Stoscheck, C.M. 1990. Meth. Enzymol. 182: 50-68. PMID: 2314256

Usage :
• Recommended working concentration: 10-25 mM

• A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with sodium hydroxide or potassium hydroxide to the pH desired. Alternatively, equimolar concentrations of HEPES (sc-29097) and of sodium HEPES can be mixed in approximately equal volumes, back-titrating with either solution to the appropriate pH.

• This product is a high quality reagent, but has not been evaluated for use in cell culture. Please employ appropriate contaminant testing and/or sterilization techniques for the intended application.
Physical State :
Solid
Solubility :
Soluble in water (500 mg/ml).
Storage :
Store at room temperature
Refractive Index :
n20D ~1.54 (Predicted)
pK Values :
pKa: 7.5 (25°C)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
2724248
MDL Number :
MFCD00036463
EC Number :
278-169-7
SMILES :
C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]

Download SDS (MSDS)

Certificate of Analysis

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HEPES Sodium salt  Product Citations

See how others have used HEPES Sodium salt. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 16531090  Guimarães, ET. et al. 2006. Microbes Infect. 8: 1219-26.

Citations 1 to 1 of 1 total
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Rated 5 out of 5 by from Various publications cite the use of HEPES Various publications cite the use of HEPES Sodium salt (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt) in the preparation of HEPES buffer, which has been described as one of the best all-purpose buffers available for use in biological research. -SCBT Publication Review
Date published: 2015-04-08
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