HEPES Sodium salt (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt) may be used in the preparation of HEPES buffer, which has been described as one of the best all-purpose buffers available for use in biological research. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5.
1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950 2. Medzon, E.L. and Gedies, A. 1971. Can. J. Microbiol. 17: 651-653. PMID: 5104240 3. Good, N.E. and Izawa, S. 1972. Meth. Enzymol. 24: 53-68. PMID: 4206745 4. Himmel, H.M., et al. 1987. J. Clin. Chem. Clin. Biochem. 25: 909-913. PMID: 3127532 5. Stoscheck, C.M. 1990. Meth. Enzymol. 182: 50-68. PMID: 2314256
• Recommended working concentration: 10-25 mM
• A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with sodium hydroxide or potassium hydroxide to the pH desired. Alternatively, equimolar concentrations of HEPES (sc-29097) and of sodium HEPES can be mixed in approximately equal volumes, back-titrating with either solution to the appropriate pH.
• This product is a high quality reagent, but has not been evaluated for use in cell culture. Please employ appropriate contaminant testing and/or sterilization techniques for the intended application.
Soluble in water (500 mg/ml).
Store at room temperature
n20D ~1.54 (Predicted)
pKa: 7.5 (25°C)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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HEPES Sodium salt Product Citations
See how others have used HEPES Sodium salt. Click on the entry to view the PubMed entry
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Various publications cite the use of HEPESVarious publications cite the use of HEPES Sodium salt (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt) in the preparation of HEPES buffer, which has been described as one of the best all-purpose buffers available for use in biological research. -SCBT Publication Review
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