Anti-Hemogen Antibody (D-4) recommended for detection of Hemogen of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Hemogen Antibody (D-4): sc-393386

Anti-Hemogen Antibody (D-4) is rated 3.0 out of 5 by 2.
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Datasheets

    • Hemogen Antibody (D-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of Hemogen of human origin
    • recommended for detection of Hemogen of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Hemogen Antibody (D-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Hemogen Antibody (D-4) (see ordering information below).
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Can Hemogen (D-4): sc-393386 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, Hemogen (D-4): sc-393386 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from Nothing is detected This antibody detected no band at the right position except many background bands. The mRNA is induced substantially. Livers are from infected mice.
Date published: 2021-06-04
Rated 5 out of 5 by from Produced positive Western blot data of Hemogen Produced positive Western blot data of Hemogen expression in K-562, Jurkat, HEL 92.1.7 and MEG-01 nuclear extracts. -SCBT QC
Date published: 2014-11-21
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