Anti-Hemogen Antibody (C-7) recommended for detection of Hemogen of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Hemogen Antibody (C-7): sc-393416

Anti-Hemogen Antibody (C-7) is rated 5.0 out of 5 by 1.
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Datasheets

    • Hemogen Antibody (C-7) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of Hemogen of human origin
    • Anti-Hemogen Antibody (C-7) is recommended for detection of Hemogen of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Hemogen Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Hemogen (C-7): sc-393416.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Hemogen Antibody (C-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Hemogen Antibody (C-7) (see ordering information below).
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Can Hemogen (C-7): sc-393416 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, Hemogen (C-7): sc-393416 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Good Western blot data of Hemogen expression in K Good Western blot data of Hemogen expression in K-562 and Jurkat nuclear extracts. -SCBT QC
Date published: 2013-06-20
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