Heme Oxygenase 1 Antibody (A-3) recommended for detection of Heme Oxygenase 1 of human origin by WB, IP, IF, IHC(P) and ELISA

Heme Oxygenase 1 Antibody (A-3): sc-136960

Heme Oxygenase 1 Antibody (A-3) is rated 4.5 out of 5 by 41.
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Datasheets

    • Anti-Heme Oxygenase 1 Antibody (A-3) is a mouse monoclonal IgG1 (kappa light chain) Heme Oxygenase 1 antibody provided at 200 µg/ml
    • raised against amino acids 184-288 of Heme Oxygenase 1 of human origin
    • Heme Oxygenase 1 Antibody (A-3) is recommended for detection of Heme Oxygenase 1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Heme Oxygenase 1 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Heme Oxygenase 1 (A-3): sc-136960.

Will this HO-1 antibody react with rat? I am trying to perform IHC (F) on rat brain sections.

Asked by: Agnes93
Thank you for your question. Heme Oxygenase 1 Antibody (A-3) is only recommended for detection of Heme Oxygenase 1 of human origin by WB, IP, IF, IHC(P) and ELISA. Based on our lab's tests we do not recommend it for rat cells or tissues. Instead you could opt for Heme Oxygenase 1 Antibody (F-4): sc-390991, which is recommended for detection of Heme Oxygenase 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.
Answered by: Tech Support Europe
Date published: 2020-10-08

How can Heme Oxygenase 1 (A-3): sc-136960 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Heme Oxygenase 1 (A-3): sc-136960 is currently available conjugated to phycoerythrin (sc-136960 PE), fluorescein (sc-136960 FITC), Alexa Fluor® 488 (sc-136960 AF488) or Alexa Fluor® 647 (sc-136960 AF647)
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 2 out of 5 by from good performance This antibody recognizes a unique band in SiHacell line and it shows good performance in total extract when cells are treated with oxidative stress inducers (i.e. Dietilmaleate/DEM)***
Date published: 2020-10-06
Rated 4 out of 5 by from Good Antibody This antibody gave a good and excellent band on Western blot.
Date published: 2020-05-08
Rated 4 out of 5 by from Excellent. This antibody works great for western blotting.Excellent.
Date published: 2019-07-30
Rated 5 out of 5 by from Great for IF and WB I received a trial size, worked well for WB and IF. Bought a full size
Date published: 2019-01-16
Rated 5 out of 5 by from SC-136960 The antibody has specific binding affinity and performs great reproducibility.
Date published: 2019-01-02
Rated 5 out of 5 by from works great! This antibody works fantastic for the western blot!
Date published: 2018-06-25
Rated 1 out of 5 by from Did not wok I obtained a free sample from the SCBT but it gave me no band of HO1 at the designated molecular weight
Date published: 2018-04-02
Rated 5 out of 5 by from perfect antibody very specific band, strong signal in human cell samples
Date published: 2017-10-24
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