Heme Oxygenase 1 Antibody (23) recommended for detection of Heme Oxygenase 1 of human origin by WB and IF

Heme Oxygenase 1 Antibody (23): sc-136256

Heme Oxygenase 1 Antibody (23) is rated 4.6 out of 5 by 5.
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Datasheets

    • Heme Oxygenase 1 Antibody (23) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 150-286 of Heme Oxygenase 1 of human origin
    • recommended for detection of Heme Oxygenase 1 of human origin by WB and IF; not recommended for IP
    • See Heme Oxygenase 1 (A-3): sc-136960 for Heme Oxygenase 1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Heme Oxygenase 1 (23): sc-136256.

What is the recommended fixation method for immunofluorescence staining with Heme Oxygenase 1 (23): sc-136256 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from ottimo segnale ! Sto usando questo anticorpo da un pò di tempo e ottengo sempre ottimi risultati
Date published: 2020-06-09
Rated 5 out of 5 by from Great antibody for western Our lab got several antibodies in October 2017, I tested 7 of them, all of them works great, For the HO-1, I tried with A2780 cells, 50uM hemin treatment. The band is pretty clear.
Date published: 2018-01-31
Rated 5 out of 5 by from Beautiful IF data This clone has beautiful IF staining in HeLa cells, however an alternate antibody to this target, Heme Oxygenase 1 (A-3): sc-136960, is the more popular choice with strong in-house data in multiple applications, and over 20 product citations.
Date published: 2017-02-01
Rated 4 out of 5 by from Produced good immunofluorescence cytoplasmic Produced good immunofluorescence cytoplasmic staining in HeLa cells. -SCBT QC
Date published: 2015-04-19
Rated 4 out of 5 by from Clean Clean, strong band in western blot analysis of Heme Oxygenase 1 expression in SW13 whole cell lysate. -SCBT QC
Date published: 2015-01-31
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