Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HELIC2 Double Nickase Plasmid (m): sc-435793-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HELIC2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HELIC2 Double Nickase Plasmid (m) and HELIC2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Snrnp200. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HELIC2 Antibody (G-9): sc-393170
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HELIC2 Double Nickase Plasmid (m) | sc-435793-NIC | 20 µg | $410.00 | |||
HELIC2 Double Nickase Plasmid (m2) | sc-435793-NIC-2 | 20 µg | $410.00 |
Snrnp200 encodes the mouse HELIC2 protein, an essential DExD/H-box RNA helicase within the U5 small nuclear ribonucleoprotein that supports spliceosome activation and catalytic steps of pre-mRNA splicing. By remodeling RNA–protein interactions during splice-site recognition and exon ligation, HELIC2 helps maintain transcriptome fidelity, coupling splicing dynamics to broader RNA processing and gene expression control. Disruption of core spliceosomal helicases can drive widespread alternative splicing changes and cellular stress responses, making Snrnp200 a useful entry point for studying RNA metabolism and genotype-to-phenotype relationships in mammalian systems. Functional interrogation of HELIC2 is also relevant for modeling mechanisms by which spliceosome perturbation contributes to disease-associated mis-splicing and altered proteostasis in experimental contexts.
HELIC2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Snrnp200 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Snrnp200. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Snrnp200 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Snrnp200-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.