HeLa nuclear extract suitable for use in Gel Shift and Western Blotting assays

HeLa nuclear extract: sc-2120

HeLa nuclear extract is rated 5.0 out of 5 by 2.
  • y_2020, m_7, d_5, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.10
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_2120, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 122ms
  • REVIEWS, PRODUCT
5
2
4
0
3
0
2
0
1
0

Datasheets

    • supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
    • provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
    • human nuclear extract; epithelioid carcinoma cells
    • suitable for use in Gel Shift and Western Blotting assays
    • Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
    • prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475

Where could I find the protocol of how these HeLa nuclear extract been generated? Thanks

Asked by: Nike_CA
Thank you for your question. Our HeLa nuclear extract was prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475.
Answered by: Technical Support
Date published: 2019-03-12

how much should be loaded for Western blot as a positive control?

Asked by: KevinW
Thank you for your question. We recommend loading 10-20 ug of this nuclear extract as a positive control for Western blotting.
Answered by: Technical Support
Date published: 2019-01-26
  • y_2020, m_7, d_4, h_15CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.10
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_2120, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 355ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from This extracted worked well as a positive control This extracted worked well as a positive control. We used it as a positive for BRCA1.
Date published: 2015-09-22
Rated 5 out of 5 by from Works well as a positive control by Western Blot Works well as a positive control by Western Blot, Would like to be able to load less than recommended 50 g protein on well. Explained that we use 50 g for testing, she can use less, but we would recommend using more if problems arise.
Date published: 2015-05-05
  • y_2020, m_7, d_5, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.10
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_2120, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 367ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.