Anti-HDGFL1 Antibody (S-18) recommended for detection of HDGFL1 of mouse origin by WB and ELISA

Anti-HDGFL1 Antibody (S-18): sc-74088

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Anti-HDGFL1 Antibody (S-18) is rated 1.0 out of 5 by 1.
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Datasheets

    • Anti-HDGFL1 Antibody (S-18) is a rat monoclonal IgG2b HDGFL1 antibody, cited in 2 publications, provided at 100 µg/ml
    • raised against full length recombinant HDGFL1 of mouse origin
    • recommended for detection of HDGFL1 of mouse origin by WB and ELISA
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HDGFL1 Antibody (S-18). This work is in progress.
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How much sample do you recommend loading on the gel for Western blot detection using HDGFL1 (S-18): sc-74088?

Asked by: jenniferc
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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