HDAC3 Antibody (40) recommended for detection of HDAC3 of mouse, rat, human and canine origin by WB, IP and IF

HDAC3 Antibody (40): sc-136290

HDAC3 Antibody (40) is rated 4.3 out of 5 by 3.
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Datasheets

    • HDAC3 Antibody (40) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 309-425 of HDAC3 of human origin
    • recommended for detection of HDAC3 of mouse, rat, human and canine origin by WB, IP and IF
    • See HDAC3 (A-3): sc-376957 for HDAC3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using HDAC3 (40): sc-136290 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenni
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Worked well in WB in human endothelial tissue Worked well in WB in human endothelial tissue samples. -SCBT QC
Date published: 2015-04-05
Rated 4 out of 5 by from Strong WB bands in HeLa nuclear extract Strong WB bands in HeLa nuclear extract. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Strong nuclear localization in human endothelial Strong nuclear localization in human endothelial cells in IF -SCBT QC
Date published: 2013-08-01
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