Anti-HB-EGF Antibody (H-1) recommended for detection of precursor HB-EGF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-HB-EGF Antibody (H-1): sc-365182

Anti-HB-EGF Antibody (H-1) is rated 4.7 out of 5 by 7.
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Datasheets

    • HB-EGF Antibody (H-1) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 183-213 within a C-terminal cytoplasmic domain of HB-EGF of mouse origin
    • Anti-HB-EGF Antibody (H-1) is recommended for detection of precursor HB-EGF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-HB-EGF Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HB-EGF (H-1): sc-365182.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for HB-EGF Antibody (H-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with HB-EGF Antibody (H-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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I would like to ask if we could obtain a trial size sample of HB-EGF Antibody (H-1): sc-365182 to see if we have success in detecting this protein using IHC-P and WB in our human tissue.

Asked by: WBNOTHNICK
Thank you for your question. For a limited time, we are currently offering a free 10µg sample of our mouse monoclonal, HB-EGF Antibody (H-1): sc-365182. This antibody has been validated to work in WB and IHC(P) with human samples. Please contact our Technical Service Department to request a sample. Please call us toll-free at (800) 457-3801 option 2, email [email protected], or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-03-07

What application is the blocking peptide sc-365182 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from 条带显示丰度不足 2月前订购HB-EGF抗体(H-1),每次实验发现该条带显示丰度不足!可能与此蛋白为膜蛋白有关系!细胞总量不够,所以要加大细胞培养数量!
Date published: 2017-10-11
Rated 5 out of 5 by from Works great at ratio 1:500 Tested it for two times. It didn't work at the first time but it worked at the second time.
Date published: 2017-08-30
Rated 5 out of 5 by from in the Western Blot Large bands observed by Western Blot at the expected molecular weight for HB-EGF protein using PC-12 whole cell lysate.
Date published: 2017-03-18
Rated 5 out of 5 by from Nice western blot from rat heart We used this antibody for western blot staining and got a nice western blot at the correct molecular weight in rat heart tissue
Date published: 2017-01-27
Rated 5 out of 5 by from Western blot A positive result was obtained in Western blot analysis,The results are shown in the following figure
Date published: 2016-11-19
Rated 5 out of 5 by from Positive results in western blot analysis Positive results in western blot analysis of human recombinant HB-EGF fusion protein. -SCBT QC
Date published: 2015-06-16
Rated 5 out of 5 by from Produced beautiful immunoperoxidase membrane Produced beautiful immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-05-10
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