Anti-HB-EGF Antibody (E-10) recommended for detection of precursor and mature HB-EGF of human origin by WB, IP, IF and ELISA

Anti-HB-EGF Antibody (E-10): sc-74526

Anti-HB-EGF Antibody (E-10) is rated 5.0 out of 5 by 3.
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Datasheets

    • HB-EGF Antibody (E-10) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 121-208 mapping at the C-terminus of mature HB-EGF of human origin
    • recommended for detection of precursor and mature HB-EGF of human origin by WB, IP, IF and ELISA
    • See HB-EGF (G-11): sc-74441 for HB-EGF antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for HB-EGF Antibody (E-10) for WB applications. These reagents are now offered in bundles with HB-EGF Antibody (E-10) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-74526: HB-EGF (E-10) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Good for WB The HB-EGF Antibody (E-10): sc-74526 provides good data for Western blot, showing clear band with minimal background. This antibody has also been cited several times for further verification.
Date published: 2017-01-25
Rated 5 out of 5 by from This antibody worked well for our WB This antibody worked well for our WB.
Date published: 2015-09-11
Rated 5 out of 5 by from WB publishable data with human HMEC WB publishable data with human HMEC - SCBT Publication Review
Date published: 2015-03-30
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