HADHSC Antibody (A-5)recommended for detection of HADHSC of mouse, rat and human origin by WB, IP, IF and ELISA

HADHSC Antibody (A-5): sc-376525

HADHSC Antibody (A-5) is rated 5.0 out of 5 by 3.
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Datasheets

    • HADHSC Antibody (A-5) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 151-314 mapping at the C-terminus of HADHSC of human origin
    • HADHSC Antibody (A-5) is recommended for detection of HADHSC of mouse, rat and human origin by WB, IP, IF and ELISA
    • HADHSC Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HADHSC (A-5): sc-376525.
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What is the recommended fixation method for immunofluorescence staining with HADHSC (A-5): sc-376525 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Good for mouse tissue It gave a clean band of the right size using mouse primary tissue lysate
Date published: 2018-12-04
Rated 5 out of 5 by from Great immunofluorescence mitochondrial staining Great immunofluorescence mitochondrial staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-26
Rated 5 out of 5 by from Great Western Blot data of HADHSC expression Great Western Blot data of HADHSC expression in non-transfected and human HADHSC transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-05-15
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