Anti-HA-Tag Antibody (F-7) recommended for detection of proteins containing the HA tag by WB, IP, IF, FCM and ELISA

Anti-HA-Tag Antibody (F-7): sc-7392

Anti-HA-Tag Antibody (F-7) is rated 4.6 out of 5 by 122.
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Datasheets

    • Anti-HA-Tag Antibody (F-7) is a mouse monoclonal IgG2a κ HA-Tag antibody, cited in 2,726 publications, provided at 200 µg/ml
    • specific to epitope mapping within an internal region of the the influenza hemagglutinin (HA) protein protein
    • HA-Tag Antibody (F-7) is recommended for detection of proteins containing the HA tag by WB, IP, IF, FCM and ELISA
    • Anti-HA-Tag Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC, PerCP or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • TransCruz reagent for ChIP application (sc-7392 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HA-Tag (F-7): sc-7392.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for HA-Tag Antibody (F-7) for WB applications. These reagents are now offered in bundles with HA-Tag Antibody (F-7) (see ordering information below).
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Hello, For the WB detection, how many hours of treatment with 1:1000 dilution do you recommend?

Asked by: Lola322
Thank you for your question. We recommend incubating over night at +4 °C. Depending on your samples and experimental setup different dilutions may produce better results on your western blots.
Answered by: Tech Support Europe
Date published: 2020-12-16

sample을 받아서 사용해볼 수 있을까요? -연세대학교 생명공학과

Asked by: Yeseul Choi
Thank you for your question,You can contact your local distributor to request SCBT antibody sample. Customers in Korea may purchase our products through one of our authorized local distributors. Your local distributor(s):Essence Medical Inc. and Koram Biotech Corporation.Alternatively, please contact our Technical Service Department to inquire about the purity of our most recent lot. Please call us directly at  (86 21) 6093-6350, email [email protected], or contact us by live chat.
Answered by: Technical Service 11
Date published: 2020-04-29

Hi there, I've purchased the mouse monoclonal anti-HA antibody but I can't find info on what the antibody is resuspended in when I buy it. What is the solvent that the antibody is in?

Asked by: The Scientist
Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Tech Service
Date published: 2020-01-21

Hi We are using this anti-HA tag antibody for using Chip assay (F-7X). But recently we have schedule to Co-IP assay for HA tag protein capture at protein G agarose bead (sc-2003). So, can we use this F-7X antibody for Co-IP assay?

Asked by: Swan
Thank you for your question. Yes, this HA-probe antibody, sc-7392, is fine for Co-IP applications.
Answered by: Tech Service
Date published: 2020-01-06

I would like to know if this ab can detect Y Y D V L D Y A or Y Y D V P D Y A via western blot.

Asked by: andriamil
Thank you for your question. The exact epitope of this antibody has not been mapped yet so it is not yet possible to say which motif is recognized. Feel free to contact our technical support directly to arrange for a sample of this antibody to find out whether it is suitable for your application.
Answered by: Technical Support Europe
Date published: 2019-04-23

Does the HA antibody work better in milk or BSA?

Asked by: corgibot
Thank you for your question. We recommend using 5% nonfat milk in TBST (0.05% Tween-20) for blocking, and for incubating both primary and secondary antibodies. You can also purchased this prepared blocking buffer on our website. https://www.scbt.com/scbt/product/tbs-blotto-a
Answered by: Technical Support
Date published: 2019-03-14

What is the storage buffer for the F-7 AC conjugated to agarose beads?

Asked by: steveb37
Thank you for your question. The agarose conjugated antibody, sc-7392 AC, is in PBS with < 0.1% sodium azide.
Answered by: Technical Support
Date published: 2019-02-02

Which are the residues ( aa) of the HA recognize by the HA-probe (F-7): sc-7392 antibody?

Asked by: Endika
Thank you for your question. The amino acid sequence of HA tag which the antibody recognizes is YPYDVPDYA.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-07
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Rated 5 out of 5 by from perfect HA antibody this antibody works well in immunofluorescence, Western blotting and ChIP
Date published: 2022-03-04
Rated 5 out of 5 by from perfect HA antibody this antibody works well in immunofluorescence, Western blotting and ChIP
Date published: 2022-03-04
Rated 5 out of 5 by from Fantastic antibody for WB, IF and IP Safe choice for WB, IF and IP, very little amount needed for any of the applications.
Date published: 2022-01-28
Rated 4 out of 5 by from 良い抗体 HAタグ付きタンパク質を安定発現させた培養細胞を使ってWBとIFを行ったところ、HAをきちんと検出できました。
Date published: 2021-05-18
Rated 5 out of 5 by from excellent antibody have been using for 15 years now. excellent for WB, IP
Date published: 2021-05-06
Rated 5 out of 5 by from excellent antibody have been using this antibody over 5 years. works excellent
Date published: 2021-01-27
Rated 4 out of 5 by from works for wb It works well for wb in HK2 cells. I'll try it also for IF
Date published: 2020-10-07
Rated 3 out of 5 by from Great antibody for western Have got free sample of this antibody. Great antibody for western on cells.
Date published: 2019-10-09
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