Anti-HA-Tag Antibody (4C12) recommended for detection of proteins containing the HA tag by WB, IP and IF

Anti-HA-Tag Antibody (4C12): sc-57594

Anti-HA-Tag Antibody (4C12) is rated 4.3 out of 5 by 3.
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Datasheets

    • HA-Tag Antibody (4C12) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
    • raised against wall antigens from the mycelial form of a common C. albicans serotype A laboratory strain
    • recommended for detection of proteins containing the HA tag by WB, IP and IF
    • See HA-Tag (F-7): sc-7392 for HA-Tag antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HA-Tag Antibody (4C12) for WB applications. These reagents are now offered in bundles with HA-Tag Antibody (4C12) (see ordering information below).
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What is the recommended secondary for HA-probe (4C12): sc-57594 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-03-07

Does anyone use goat-anti-mouse IgG-HRP conjugate(sc-2005), and successfully find a band? (I used 1:200 dilution of primary Ab and 1:5000 of secondary Ab.)

Asked by: FYT0429
Thank you for your question. We have successfully used this mouse monoclonal IgG1 primary antibody, HA-probe (4C12): sc-57594, together with goat anti-mouse IgG-HRP conjugated secondary antibody (sc-2005). We would expect a signal using the same dilutions that you have tested. Please contact our Technical Service Department and we would be happy to assist you in troubleshooting these antibodies. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat. We would also recommend trying our recombinant mouse IgGκ binding protein alternatives to this conventional secondary anti-mouse IgG reagents. The product number is m-IgGκ BP-HRP: sc-516102.
Answered by: Technical Support
Date published: 2016-12-02
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Rated 5 out of 5 by from Nice antibody for western blot detection We used this antibody for western blot expression. We got a perfect band at the correct molecular weight
Date published: 2017-01-31
Rated 4 out of 5 by from Nice specific single bands on the WB Nice specific single bands on the WB. -SCBT QC
Date published: 2014-04-22
Rated 4 out of 5 by from We used this a few times and saw a strong We used this a few times and saw a strong protein band, but there was some background. -SCBT QC
Date published: 2013-06-02
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