Thank you for your question. We have successfully used this mouse monoclonal IgG1 primary antibody, HA-probe (4C12): sc-57594, together with goat anti-mouse IgG-HRP conjugated secondary antibody (sc-2005). We would expect a signal using the same dilutions that you have tested. Please contact our Technical Service Department and we would be happy to assist you in troubleshooting these antibodies. Please call our Asian Support office toll-free at 800-820-8626, email [email protected]
, or contact us by live chat.
We would also recommend trying our recombinant mouse IgGκ binding protein alternatives to this conventional secondary anti-mouse IgG reagents. The product number is m-IgGκ BP-HRP: sc-516102.
Date published: 2016-12-02