Guanidine Hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids, especially mRNA. Guanidine Hydrochloride is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins and isolation of RNA. At higher concentrations, this strong denaturant has been reported to be able to solubilize denatured insoluble proteins such as inclusion bodies. When utilized at lower concentrations, Guanidine Hydrochloride is noted to be useful in prompting the refolding of denatured proteins and had been reported to allow return of enzymatic activity in protocols using Guanidine Hydrochloride as a first step.
1. Pace, C.N., et al. 1990. Biochemistry. 29: 2564-2572. PMID: 2110472
2. Marston, F.A., et al. 1990. Meth. Enzymol. 182: 264-276. PMID: 2179665
3. Levine, A.D., et al. 1995. J. Biol. Chem. 270: 7445-7452. PMID: 7706290
4. Rudolph, R. and Lilie, H. 1996. FASEB J. 10: 49-56. PMID: 8566547
5. Mukhopadhyay, A. 1997. Adv. Biochem. Eng. Biotechnol. 56: 61-109. PMID: 8939059
See how others have used Guanidine Hydrochloride (CAS 50-01-1). Click on the entry to view the PubMed entry .
PMID: # 33665237 Ustriyana, P.|Schulte, F.|Gombedza, F.|Gil-Bona, A.|Paruchuri, S.|Bidlack, FB.|Hardt, M.|Landis, WJ.|Sahai, N.| et al. 2021. Bone Rep. 14: 100754.