Anti-GST Antibody (A-6) recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA

Anti-GST Antibody (A-6): sc-374171

Anti-GST Antibody (A-6) is rated 5.0 out of 5 by 6.
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Datasheets

    • GST Antibody (A-6) is a mouse monoclonal IgG1 κ, cited in 31 publications, provided at 200 µg/ml
    • raised against a sequence of GST of S. japonicum origin
    • GST Antibody (A-6) is recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA
    • Anti-GST Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GST (A-6): sc-374171.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GST Antibody (A-6) for WB applications. These reagents are now offered in bundles with GST Antibody (A-6) (see ordering information below).
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Can I dilute this antibody in milk?

Asked by: Chuma2011
Thank you for your question. Yes, you can dilute this antibody in 5% milk for Western blot use.
Answered by: Technical Support
Date published: 2019-01-26

What secondary is best to use with GST (A-6): sc-374171 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with GST (A-6): sc-374171, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-12

At what molecular weight should the band be seen in Western Blot with GST (A-6): sc-374171 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. The molecular weight expected varies depending on the fusion protein you are trying to detect. It should equal to the protein of interest plus the weight of the GST tag.
Answered by: Technical Support 10
Date published: 2017-01-11
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Rated 5 out of 5 by from very nice signal in western blot! used in western blot to detect GST-tagged proteins in budding yeast total protein extract - worked very well at 1:5000 dilution
Date published: 2018-02-08
Rated 5 out of 5 by from Strong WB !! Very nice western blot data;strong signal, low background
Date published: 2017-01-28
Rated 5 out of 5 by from Strong WB data The GST Antibody (A-6): sc-374171 provides good Western blot data showing clear band with minimal background.
Date published: 2017-01-11
Rated 5 out of 5 by from Worked well for WB on human CNE2 and DDP Worked well for WB on human CNE2 and DDP cell lysates. -SCBT Publication Review
Date published: 2015-04-15
Rated 5 out of 5 by from Published ChIP data using A Published ChIP data using A-6 on mouse thymocyte extracts. -SCBT Publication Review
Date published: 2015-01-17
Rated 5 out of 5 by from Strong signal observed in Western blot analysis Strong signal observed in Western blot analysis of S. japonicum recombinant GST fusion protein. -SCBT QC
Date published: 2013-12-11
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