Anti-GST Antibody (1E5) recommended for detection of GST by WB and IP

Anti-GST Antibody (1E5): sc-53909

Anti-GST Antibody (1E5) is rated 4.5 out of 5 by 8.
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Datasheets

    • GST Antibody (1E5) is a mouse monoclonal IgG2b, cited in 28 publications, provided at 50 µg/500 µl
    • raised against recombinant Glutathione S-transferase
    • recommended for detection of GST by WB and IP
    • See GST (B-14): sc-138 for GST antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GST (1E5): sc-53909.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for GST Antibody (1E5) for WB applications. These reagents are now offered in bundles with GST Antibody (1E5) (see ordering information below).
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 5 out of 5 by from Excellent for WB The (1E5) clone is well established and a popular choice for detection of GST fusion proteins in WB.
Date published: 2017-01-07
Rated 5 out of 5 by from Helpful! I used this GST antibody in my WB assay and recieved excellent result. It can indentify my GST tagged fusion protein specifically. So I think its a good product.
Date published: 2016-12-31
Rated 5 out of 5 by from GST antibody in WB I have already used this GST antibody in my WB assay and recieved excellent result. It can indentify my GST tagged fusion protein specifically.
Date published: 2016-11-24
Rated 1 out of 5 by from Does not work in Western Blot Does not work in Western Blot.
Date published: 2015-03-31
Rated 5 out of 5 by from Good results with PC Good results with PC-3, DU-145 and LNCaP cell lines in WB -SCBT Publication Review
Date published: 2015-02-19
Rated 5 out of 5 by from Used for WB with PC Used for WB with PC-3, MDCK and COS-7 cell lines -SCBT Publication Review
Date published: 2014-01-09
Rated 5 out of 5 by from Detects recombinant GST and GST Detects recombinant GST and GST-tagged proteins in WB -SCBT QC
Date published: 2013-12-13
Rated 5 out of 5 by from Worked in WB with transfected 293T cells Worked in WB with transfected 293T cells -SCBT Publication Review
Date published: 2013-05-21
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