GSK-3 beta CRISPR Plasmids (m) mouse GSK-3β-specific CRISPR/Cas9 KO Plasmid, HDR Plasmid, Double Nickase Plasmids, CRISPR Activation Plasmids and Lentiviral Activation Particles

GSK-3β CRISPR Plasmids (m)

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Datasheets

    • Target species: mouse; for corresponding human product, see GSK-3β CRISPR Plasmids (h)
    • CRISPR/Cas9 KO Plasmids consists of GSK-3β-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
    • For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
    • Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
    • Gene knockdown or activation can be assayed using GSK-3β Antibody (E-11): sc-377213
    • All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells

Gene Info

SpeciesGene NameGene IDChromosome LocationIsoform (mRNA) Accession #Protein Accession #OMIM™ Number
MouseGsk3b5663716 B3XM_006522425, XM_006522426, NM_019827, NM_001347232Q9WV60N/A
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Is CRSPR activation lentiviral  applicable for in vivo animal infection? I think the protocol says it is for cultured cells but i can’t find any reason (for that your shRNA lenti worked beautifully in vivo) Can you share your speculation or refs?

Asked by: Kungfumaster5132
Thank you for your question. We do not recommend nor support the use of our CRISPR Lentiviral Activation Particles for in vivo research applications. One possible consideration is that the viral titer may be too low (we provide 200 uL containing 1e6 particles). In addition, the Lentiviral Activation Particles encode a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells (see the reference below for more information). We do not have any data or reference using these for in vivo applications. Konermann, S., et al. 2015. Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 517: 583-588. PMID 25494202
Answered by: Technical Support
Date published: 2019-05-01
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