Anti-GSK-3 beta Antibody (2Q274) recommended for detection of GSK-3β of human, rat and Xenopus origin by WB and IP

Anti-GSK-3β Antibody (2Q274): sc-71188

Anti-GSK-3β Antibody (2Q274) is rated 4.0 out of 5 by 3.
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Datasheets

    • GSK-3β Antibody (2Q274) is a mouse monoclonal IgG2a, cited in 1 publications, provided at 50 µg/0.5 ml
    • raised against recombinant GSK-3β of Xenopus laevis origin
    • recommended for detection of GSK-3β of human, rat and Xenopus origin by WB and IP
    • See GSK-3α/β (0011-A): sc-7291 for GSK-3β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GSK-3β Antibody (2Q274). This work is in progress.
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I am using GSK-3β (2Q274): sc-71188 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Multiple bands seen in WB corresponding to GSK Multiple bands seen in WB corresponding to GSK-3 in HeLa, A549, SK-BR-3 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-02-28
Rated 4 out of 5 by from Multiple bands seen in WB corresponding to GSK Multiple bands seen in WB corresponding to GSK-3 in HeLa, A549, SK-BR-3 and Jurkat whole cell lysates. -SCBT QC
Date published: 2013-02-05
Rated 4 out of 5 by from Strong WB signal seen in human GSK Strong WB signal seen in human GSK-3 transfected 293T whole cell lysate. -SCBT QC
Date published: 2013-02-05
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