Anti-GSK-3 beta Antibody (11B9) recommended for detection of GSK-3β of mouse, rat and human origin by WB and IP

Anti-GSK-3β Antibody (11B9): sc-81462

Anti-GSK-3β Antibody (11B9) is rated 4.3 out of 5 by 7.
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Datasheets

    • GSK-3β Antibody (11B9) is a mouse monoclonal IgG1, cited in 66 publications, provided at 50 µg/0.5 ml
    • raised against the N-terminus of GSK-3β of human origin
    • recommended for detection of GSK-3β of mouse, rat and human origin by WB and IP; also reactive with additional species, including canine
    • See GSK-3α/β (0011-A): sc-7291 for GSK-3β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GSK-3β (11B9): sc-81462.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GSK-3β Antibody (11B9) for WB applications. These reagents are now offered in bundles with GSK-3β Antibody (11B9) (see ordering information below).
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What is the recommended dilution to use GSK-3β (11B9): sc-81462 monoclonal antibody in Western blot?

Asked by: cjMara
We recommend using GSK-3β (11B9): sc-81462 monoclonal antibody at a starting dilution of 1:200 (range 1:100-1:1,000) for Western blotting.
Answered by: Technical Support 9
Date published: 2017-01-26
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Rated 5 out of 5 by from Nice antibody Specific for gsk3 beta at 1:500. May be even good to use at 1:1000 but we just never tried. This cell line is HEK293.
Date published: 2018-11-28
Rated 5 out of 5 by from strong signal Strong signal observed by WB with GSK-3 beta Antibody (11B9)
Date published: 2017-01-24
Rated 4 out of 5 by from Published data for WB in mouse R390A c Published data for WB in mouse R390A c-src and D338N c-src cell lines. -SCBT Publication Review
Date published: 2015-05-19
Rated 4 out of 5 by from Worked well in WB in human GSK Worked well in WB in human GSK-3 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-05-18
Rated 4 out of 5 by from Published data for IP and WB in mouse kidney Published data for IP and WB in mouse kidney proximal tubular epithelial cells. -SCBT Publication Review
Date published: 2015-04-04
Rated 4 out of 5 by from Published data for WB in rat brain Published data for WB in rat brain. -SCBT Publication Review
Date published: 2015-01-10
Rated 4 out of 5 by from Clear band in WB in mouse GSK Clear band in WB in mouse GSK-3 transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2014-02-20
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