Anti-GSK-3 alpha / beta Antibody (1H8) recommended for detection of GSK-3α and GSK-3β of mouse, rat, human and Xenopus laevis origin by WB and IP

Anti-GSK-3α/β Antibody (1H8): sc-56913

Anti-GSK-3α/β Antibody (1H8) is rated 4.0 out of 5 by 7.
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Datasheets

    • GSK-3α/β Antibody (1H8) is a mouse monoclonal IgG2a κ, cited in 20 publications, provided at 200 µg/ml
    • raised against recombinant GSK-3α/β of Xenopus laevis origin
    • recommended for detection of GSK-3α and GSK-3β of mouse, rat, human and Xenopus laevis origin by WB and IP
    • See GSK-3α/β (0011-A): sc-7291 for GSK-3α/β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GSK-3α/β Antibody (1H8) for WB applications. These reagents are now offered in bundles with GSK-3α/β Antibody (1H8) (see ordering information below).
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Does this antibody recognize the GSK3a/b fragments? Such as 30kd, and 15kd?

Asked by: Lanmio
Thank you for your question. We have not observed any fragments at 15 kDa using this antibody. Please refer to the representative Western blot data on our website.
Answered by: Technical Support
Date published: 2019-01-26

Are there any issues with using sc-56913: GSK-3α/β (1H8) mouse monoclonal primary antibody on mouse samples?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-56913 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from Good antibody For endogenous GSK3 in B-cell lymphoma cells, the signaling is quite strong!
Date published: 2018-02-26
Rated 4 out of 5 by from Large Large, strong band in WB in A6 whole cell lysate. -SCBT QC
Date published: 2015-03-10
Rated 4 out of 5 by from Published data for WB in mouse brain sample Published data for WB in mouse brain sample. -SCBT Publication Review
Date published: 2015-02-02
Rated 4 out of 5 by from Publishable data for WB Publishable data for WB, IP and IHC (P) in HeLa, HTC116 and Cos7 cell lines. -SCBT Publication Review
Date published: 2015-01-17
Rated 4 out of 5 by from Published data for WB in mouse skeletal muscle Published data for WB in mouse skeletal muscle sample. -SCBT Publication Review
Date published: 2015-01-07
Rated 4 out of 5 by from Published data for WB in human U2OS cells Published data for WB in human U2OS cells.
Date published: 2015-01-04
Rated 4 out of 5 by from Robust positive band seen in WB in Caki Robust positive band seen in WB in Caki-1, HeLa, A549, SK-BR-3, Jurkat and LNCaP whole cell lysates. -SCBT QC
Date published: 2013-07-19
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