Anti-GSK-3α/β Antibody (0011-A): sc-7291
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- Anti-GSK-3α/β Antibody (0011-A) is a mouse monoclonal IgG2a κ GSK-3α/β antibody, cited in 244 publications, provided at 200 µg/ml
- raised against amino acids 1-420 representing full length glycogen synthase kinase-3 beta of Xenopus laevis origin
- Anti-GSK-3 alpha / beta Antibody (0011-A) is recommended for detection of GSK-3α and GSK-3β of mouse, rat, human, canine and Xenopus origin by WB, IP, IF, IHC(P) and FCM
- Anti-GSK-3 alpha / beta Antibody (0011-A) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GSK-3α/β (0011-A): sc-7291.
- m-IgGκ BP-HRP is the preferred secondary detection reagent for GSK-3α/β Antibody (0011-A) for WB and IHC(P) applications. This reagent is now offered in a bundle with GSK-3α/β Antibody (0011-A) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
QUICK LINKS
SEE ALSO...
- Additional GSK Antibodies including GSK-3 β
- ChemCruz™ Chemical GSK-3 alpha Inhibitors for functional analysis of cellular responses to GSK-3 α
- Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional
Anti-GSK-3α/β Antibody (0011-A): sc-7291. Is this antibody yeast reactive?
Yeast Reactivity validation is untested and unknown.
Date published: 2021-06-25
How should the cells be fixed for flow cytometry?
Thank you for your question. We recommend using a fixation buffer to fix cells for Flow cytometry. Our buffer can be found here:
FCM Fixation Buffer (sc-3622) : https://www.scbt.com/scbt/product/fcm-fixation-buffer
Our recommended protocol is here:
https://www.scbt.com/scbt/resources/protocols/flow-cytometry
If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Date published: 2017-10-10
Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?
You definitely can. We recommend using a directly conjugated primary antibody. SC-7291 is available directly conjugated to AF488, AF647, and FITC.
Date published: 2016-12-21
What is the recommended secondary reagent to be used with this mouse monoclonal primary?
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here:
https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Date published: 2016-12-21
Rated 5 out of
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SilviaB from
Good antibody for WB
I used this antibody diluted 1:500 in milk/TBST buffer, loading 30ug of total protein. The signal is strong, clean bands without any background.
Date published: 2021-06-22
Rated 5 out of
5
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camtelee from
A clean and potent antibody
We purchased the antibody last month for quantification of GSK by Western blotting. We are able to detect both GSK isoforms from HepG2 cells using 2 mcg antibody per blot. Non-specific band was not detected. This is an excellent antibody.
Date published: 2019-10-24
Rated 5 out of
5
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Yuanhong from
good product
It works pretty well for our western blot experiment.
Date published: 2018-08-22
Rated 4 out of
5
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Agnes2004 from
Great antibodies
I got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 5 out of
5
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HappyFlower from
Great antibody for western blot
The batch of antibody I have worked well on BJAB, HeLa, MCF7, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 250 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 5 out of
5
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Mimmuz from
Super anticorps !!
J'ai tester cet échantillon en WB 1/500 et il marche super bien, signal très spécifique, sans bruit de fond (membrane strippé et réutilisé déjà 3 fois!!).
Date published: 2017-11-30
Rated 5 out of
5
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rjanostiak from
Very good - Western
Very good signal, clear bands, practically no background (Western blot, melanoma cells)
Date published: 2017-11-03
Rated 5 out of
5
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haoqing from
Results were great.
This is a good WB in MDCK (E) whole cell lysate, I diluted 1:500 with TBST, the results were stable and clear.
Date published: 2017-10-27