Anti-GSK-3 alpha Antibody (H-12) recommended for detection of GSK-3α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-GSK-3α Antibody (H-12): sc-5264

Anti-GSK-3α Antibody (H-12) is rated 4.8 out of 5 by 8.
  • y_2022, m_5, d_19, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_en_US, sid_5264, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 37ms
  • REVIEWS, PRODUCT
loading
5
6
4
2
3
0
2
0
1
0

Datasheets

    • Anti-GSK-3α Antibody (H-12) is a mouse monoclonal IgG1 κ GSK-3α antibody, cited in 13 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 464-483 at the C-terminus of GSK-3α of human origin
    • Anti-GSK-3 alpha Antibody (H-12) is recommended for detection of GSK-3α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-GSK-3 alpha Antibody (H-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GSK-3α (H-12): sc-5264.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GSK-3α Antibody (H-12) for WB and IHC(P) applications. These reagents are now offered in bundles with GSK-3α Antibody (H-12) (see ordering information below).
loading

What application is the blocking peptide sc-5264 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2022, m_5, d_19, h_21CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_5264, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 20ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from fairly good antibody I used it several times for western blot, fairly good. It has non-specific band on top.
Date published: 2018-02-27
Rated 5 out of 5 by from Nice dark band in western blot We used this antibody for detection in A-431 cells. We got a positive band when looking at the western blot
Date published: 2017-01-12
Rated 5 out of 5 by from Distinct solid band at expected molecular Distinct solid band at expected molecular weight for HeLa cells observed by WB. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Publishable data obtained by IF analysis Publishable data obtained by IF analysis of human SH-SY5Y cells. -SCBT Publication Review
Date published: 2015-04-25
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic staining Great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-03-09
Rated 4 out of 5 by from Strong signal observed by WB using A431 and Strong signal observed by WB using A431 and A549 whole cell lysates. -SCBT QC
Date published: 2015-03-09
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the GSK-3 protein via Western Blot analysis. -SCBT QC
Date published: 2015-01-22
Rated 5 out of 5 by from Numerous publications support of this antibody Numerous publications support of this antibody in Western Blot using human SH-SY5Y, U87-MG, Cal27, HCT116, HEK293T and Hep 3B cells. -SCBT Publication Review
Date published: 2015-01-04
  • y_2022, m_5, d_19, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_en_US, sid_5264, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 23ms
  • REVIEWS, PRODUCT