Anti-GSK-3 alpha Antibody (10C1) recommended for detection of GSK-3α of mouse, rat, human and canine origin by WB and IP

Anti-GSK-3α Antibody (10C1): sc-81463

Anti-GSK-3α Antibody (10C1) is rated 4.0 out of 5 by 1.
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Datasheets

    • GSK-3α Antibody (10C1) is a mouse monoclonal IgG1, cited in 1 publications, provided at 50 µg/0.5 ml
    • raised against GSK-3α of human origin
    • recommended for detection of GSK-3α of mouse, rat, human and canine origin by WB and IP; non cross-reactive with GSK-3β
    • See GSK-3α/β (0011-A): sc-7291 for GSK-3α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GSK-3α Antibody (10C1). This work is in progress.
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I am using GSK-3α (10C1): sc-81463 antibody for immunoprecipitation, how much antibody should I use?

Asked by: cjMara
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Positive band for WB in human GSK Positive band for WB in human GSK-3 transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2013-04-11
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