Anti-gp91-phox Antibody (54.1) recommended for detection of gp91-phox of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-gp91-phox Antibody (54.1): sc-130543

Anti-gp91-phox Antibody (54.1) is rated 4.9 out of 5 by 20.
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Datasheets

    • Anti-gp91-phox Antibody (54.1) is a mouse monoclonal IgG1 κ gp91-phox antibody, cited in 91 publications, provided at 200 µg/ml
    • raised against gp91-phox of human origin, with epitope mapping to amino acids 383-390
    • Anti-gp91-phox Antibody (54.1) is recommended for detection of gp91-phox of mouse, rat and human origin by WB, IP, IF and IHC(P); also recommended for detection of ERp57, Mox1, Nox3 and Nox4
    • Anti-gp91-phox Antibody (54.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of gp91-phox (54.1): sc-130543.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for gp91-phox Antibody (54.1) for WB and IHC(P) applications. These reagents are now offered in bundles with gp91-phox Antibody (54.1) (see ordering information below).
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Does this antibody work with flow cytometry?

Asked by: pinder
thank you for your question. Anti-gp91-phox Antibody (54.1) is available conjugated to either phycoerythrin or FITC for FCM, IF, and IHC(P).
Answered by: Tech Support Europe
Date published: 2021-04-07

Can I use this antibody conjugated with Phycoerythrin for IHC or IF? If yes, under which channel can I image the stained cells? Is there a protocol for IHC or IF for this antibody? Thank you

Asked by: Keertana
Thank you for your question. Anti-gp91-phox Antibody (54.1) and its conjugates are recommended for detection of gp91-phox of mouse, rat and human origin by WB, IP, IF and IHC(P). They are also recommended for detection of ERp57, Mox1, Nox3 and Nox4. You can find our recommended protocols for IHC and IF experiments under this link: https://www.scbt.com/resources/protocols PE absorption peaks in the visible light spectrum are measured at 495 and 545/566 nm.
Answered by: Technical Support Europe
Date published: 2020-10-12

Is it possible for me to have a free trial sample for the above antibody? Thank you!

Asked by: Renato
Thank you very much for your inquiry. Please send us an email to [email protected] with your complete delivery address, we will contact you back as soon as possible.
Answered by: Technical Support Europe
Date published: 2017-12-05

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-130543 PE) or fluoroscein (sc-130543 FITC), Alexa Fluor® 488 (sc-130543 AF488) or Alexa Fluor® 647 (sc-130543 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-04
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Rated 5 out of 5 by from good antibody the antibody works very well in IHC. No problem with background.
Date published: 2020-12-01
Rated 5 out of 5 by from Very good for Western Blotting Works great for western blotting (mouse heart lysate), can see clear bands at about 60 kDa.
Date published: 2019-07-05
Rated 5 out of 5 by from worked It works great - I 'd recommend this Nox2 antibody.
Date published: 2018-03-16
Rated 5 out of 5 by from Great IB antibody This antibody works great with mouse cells. We also included Nox2-KO cells as a genetic control.
Date published: 2017-10-08
Rated 4 out of 5 by from very good The strips are very clear and there are not many bands.
Date published: 2017-08-27
Rated 5 out of 5 by from IF worked in murine DC 2.4 cells It worked great with murine DC 2.4 cells. I did not try to optimize the concentration, but it worked with 1:50 as they recommended.
Date published: 2017-07-08
Rated 5 out of 5 by from This antibody work well for WB This antibody can also detect the samples from the pig ,it can replace the sc-5826 .
Date published: 2017-06-23
Rated 5 out of 5 by from Awesome in WB Great signal in WB. Detects a single band in HeLa lysate at a 1:1000 dilution.
Date published: 2017-06-06
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