GM130 Double Nickase Plasmid (h): sc-400787-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GM130 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GM130 Double Nickase Plasmid (h) and GM130 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GOLGA2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GM130 Antibody (H-7): sc-55590
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GM130 Double Nickase Plasmid (h) | sc-400787-NIC | 20 µg | $410.00 | |||
GM130 Double Nickase Plasmid (h2) | sc-400787-NIC-2 | 20 µg | $410.00 |
GOLGA2 encodes the cis-Golgi matrix protein GM130, a central scaffold for Golgi ribbon organization and vesicle tethering that supports efficient ER-to-Golgi transport and cisternal stacking. GM130 coordinates with tethering factors and small GTPase-regulated trafficking networks to maintain Golgi architecture during interphase and promote Golgi reassembly following mitosis, influencing polarized secretion and organelle positioning. Through its roles in membrane trafficking, protein processing, and cell-cycle–linked Golgi dynamics, altered GM130 function is relevant to cellular stress responses, migration phenotypes, and disease-associated perturbations in Golgi structure reported across multiple biological contexts.
GM130 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GOLGA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GOLGA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GOLGA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GOLGA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.