glypican-3 Antibody (F-3)recommended for detection of glypican-3 of mouse, rat and human origin by WB, IP, IF and ELISA

glypican-3 Antibody (F-3): sc-390587

glypican-3 Antibody (F-3) is rated 4.7 out of 5 by 3.
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Datasheets

    • glypican-3 Antibody (F-3) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 48-74 of glypican-3 of human origin
    • recommended for detection of glypican-3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • blocking peptide, sc-390587 P
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of glypican-3 (F-3): sc-390587.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Is the glypican-3 Antibody (F-3): sc-390587 (clone IG12) available in conjugation with Alexa 405 ?

Asked by: Zhouhong
Thank you for your question. glypican-3 Antibody (F-3): sc-390587 is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM. We do not provide it conjugated to AF405.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What application is the blocking peptide sc-390587 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Works for immunofluorescence on mouse cells Used on mouse ovarian epithelial cells at 1/100 overnight at 4C then 1/500 goat α-mouse secondary for 1H at room temp. Decent signal to noise ratio.
Date published: 2017-03-24
Rated 5 out of 5 by from Nice dark western blot We used this antibody for western blot and got a nice band for the correct molecular weight
Date published: 2017-01-13
Rated 5 out of 5 by from Produced positive Western blot data of glypican Produced positive Western blot data of glypican-3 expression in COLO320DM, SW480 and HTC 116 whole cell lysates. -SCBT QC
Date published: 2013-08-09
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