glypican-3 Antibody (1G12)recommended for detection of glypican-3 of human origin by WB, IP, IF and IHC(P)

glypican-3 Antibody (1G12): sc-65443

glypican-3 Antibody (1G12) is rated 5.0 out of 5 by 5.
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Datasheets

    • glypican-3 Antibody (1G12) is a mouse monoclonal IgG1 provided at 15 µg/100 µl
    • raised against a fragment containing amino acids 510-580 of glypican-3 of human origin
    • recommended for detection of glypican-3 of human origin by WB, IP, IF and IHC(P)
    • See glypican-3 (F-3): sc-390587 for glypican-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2016-12-16

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 4
Date published: 2016-12-16
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Rated 5 out of 5 by from very strong signal Nice western blot data, clear background effect is very good
Date published: 2017-05-23
Rated 5 out of 5 by from Works great in Western Blot! This antibody works great in western blot analysis of glypican-3 expression in 293T whole cell lysate.
Date published: 2016-12-16
Rated 5 out of 5 by from Works very well by IHC(P) Works very well by IHC(P) - Published data
Date published: 2015-08-04
Rated 5 out of 5 by from Worked well in WB in human glypican Worked well in WB in human glypican-3 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-01-07
Rated 5 out of 5 by from Clear Clear, strong band seen in WB in 293T whole cell lysate. -SCBT QC
Date published: 2013-06-16
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