Glycophorin A Antibody (R10)recommended for detection of Glycophorin A of human origin by WB, IP, IF, IHC(P) and FCM

Glycophorin A Antibody (R10): sc-53905

Glycophorin A Antibody (R10) is rated 4.2 out of 5 by 6.
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Datasheets

    • Glycophorin A Antibody (R10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against Abelson murine leukemia virus-induced human pre-B tumor cells
    • Glycophorin A Antibody (R10) is recommended for detection of Glycophorin A of human origin by WB, IP, IF, IHC(P) and FCM
    • Glycophorin A Antibody (R10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Glycophorin A (R10): sc-53905.
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Could you please share the protocol of the IHC staining of the second image that you posted? My humanized mice samples consist of both human and mouse rbc, will this cause any unspecific binding to mouse rbc?

Asked by: Hooi Linn
Thank you for your question. A link to our recommended protocol that was used for IHC staining with this Glycophorin A Antibody (R10): sc-53905 is provided below. This antibody is recommended for detection of Glycophorin A of human origin. We do not expect the antibody to cross-react with Glycophorin A of mouse origin, however we have not experimentally confirmed this lack of species reactivity. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-05-03

Can Glycophorin A (R10): sc-53905 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Glycophorin A (R10): sc-53905 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-04-28
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Rated 2 out of 5 by from WB We saw band for Glycopurin A (R10) in the WB of mouse lung primary fibroblast, but it was in the wrong fraction. Instead of membrane fraction, the band was in cytoplasmic fraction. We had negative and positive control for the fraction extraction. The size of the band also was not true, it was about 50 KD. We used anti-mouse IgG1.
Date published: 2018-03-28
Rated 4 out of 5 by from it worked. two month ago, i applied for this antibody and used it to detect the protein in Red blood cell sample, in generally, it worked, but instability.
Date published: 2017-03-04
Rated 5 out of 5 by from Reliable Antibody Great for IHC This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and Flow Cytometry. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-23
Rated 5 out of 5 by from Excellent immunoperoxidase membrane staining Excellent immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human blood vessel tissue. -SCBT QC
Date published: 2015-03-22
Rated 4 out of 5 by from Nice bands seen in WB using K Nice bands seen in WB using K-562 and human PBL whole cell lysates. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Excellent FCM data of human red blood cells Excellent FCM data of human red blood cells stained with Glycophorin A -SCBT QC
Date published: 2014-06-09
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