Glut1 Antibody (A-4)recommended for detection of Glut1 of human origin by WB, IP, IF, IHC(P) and ELISA

Glut1 Antibody (A-4): sc-377228

Glut1 Antibody (A-4) is rated 4.3 out of 5 by 24.
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Datasheets

    • Glut1 Antibody (A-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 218-260 of Glut1 of human origin
    • Glut1 Antibody (A-4) is recommended for detection of Glut1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Glut1 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Glut1 (A-4): sc-377228.
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What type of secondary antibodies are recommended to use for this antibody? Is it possible to use horseradish peroxidase secondary antibodies?

Asked by: Lea_
Thank you for your question. You can use any anti-mouse secondary, including HRP conjugated secondary antibodies. We recommend using our Mouse IgG Binding protein, you can find it here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2019-01-16

For immunoprecipitation, does the antibody detect dentaured or native protein?

Asked by: orangina
Thank you for your question. Glut1 Antibody (A-4): sc-377228 is recommended for detection of Glut1 of human origin by WB, IP, IF, IHC(P) and ELISA, so it detects both the denatured and the native protein.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

Hi, This product detect GLUT-1 in Mouse?

Asked by: Rigoberto
Thank you for your question. We do not currently recommend this antibody, sc-377228: Glut1 (A-4), for use in mouse samples because we have not yet tested it in mouse. The antibody is raised against a recombinant protein sequence that shares significant (95%) identity with the mouse Glut1 protein. Therefore, it is highly likely that the antibody will cross-react with mouse Glut1. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to request a free sample to test in mouse. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-12-14

Can Glut1 Antibody (A-4): sc-377228 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Glut1 Antibody (A-4): sc-377228 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 1 out of 5 by from Did not work for WB Did not see any bands for western blots for DLD-1 cell lines. I tried 1:100 and 1:500 (with 1:10,000 and 1:5000 secondary).
Date published: 2019-07-17
Rated 1 out of 5 by from kein Ergebnis beim WB Hat beim WB (1/200) mit SHSY5Y und HEP Zellen keine spezifische Bande gezeigt
Date published: 2018-04-03
Rated 4 out of 5 by from WB I got the bands and correct position by wextern blot (1:200), but hope work better.
Date published: 2018-03-01
Rated 5 out of 5 by from Very good Works very well in human neural stem cells. Visible band at 55 kDa.
Date published: 2017-12-14
Rated 4 out of 5 by from Pretty good I got a pretty clean western with this antibody. I used a 1:300 dilution and a 1:5000 secondary.
Date published: 2017-11-24
Rated 5 out of 5 by from Gute Ergebnisse im Western Blot Mit der Probegröße hatte ich gute Ergebnisse im Western Blot.
Date published: 2017-11-22
Rated 4 out of 5 by from The best It works very well in Hs578t cell line (1:500). The signal is strong.
Date published: 2017-10-31
Rated 5 out of 5 by from a good antibody The GLUT1 antibody is good for western blot and I recommended this antibody.
Date published: 2017-10-06
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