Glut1 Antibody (A-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 218-260 of Glut1 of human origin
Glut1 Antibody (A-4) is recommended for detection of Glut1 of human origin by WB, IP, IF, IHC(P) and ELISA
Glut1 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Glut1 (A-4): sc-377228.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. In adipose and muscle tissue, insulin stimulates a rapid and dramatic increase in glucose uptake, which is largely due to the redistribution of the insulin-inducible glucose transporter, Glut4. In response to insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane, and shows a much less dramatic translocation in response to insulin. Glut1 and Glut4 are twelve-pass transmembrane proteins (12TM) whose carboxy-termini may dictate their cellular localization. Aberrant Glut4 expression has been suggested to contribute to such maladies as obesity and diabetes. Glut4 null mice have shown that while functional Glut4 protein is not required for maintaining normal glucose levels, it is necessary for sustained growth, normal cellular glucose, fat metabolism and prolonged longevity.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What type of secondary antibodies are recommended to use for this antibody? Is it possible to use horseradish peroxidase secondary antibodies?
Asked by: Lea_
Thank you for your question. You can use any anti-mouse secondary, including HRP conjugated secondary antibodies. We recommend using our Mouse IgG Binding protein, you can find it here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2019-01-16
For immunoprecipitation, does the antibody detect dentaured or native protein?
Asked by: orangina
Thank you for your question. Glut1 Antibody (A-4): sc-377228 is recommended for detection of Glut1 of human origin by WB, IP, IF, IHC(P) and ELISA, so it detects both the denatured and the native protein.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29
Hi, This product detect GLUT-1 in Mouse?
Asked by: Rigoberto
Thank you for your question. We do not currently recommend this antibody, sc-377228: Glut1 (A-4), for use in mouse samples because we have not yet tested it in mouse. The antibody is raised against a recombinant protein sequence that shares significant (95%) identity with the mouse Glut1 protein. Therefore, it is highly likely that the antibody will cross-react with mouse Glut1.
For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to request a free sample to test in mouse. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-12-14
Can Glut1 Antibody (A-4): sc-377228 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Asked by: jenni
Thank you for your question. Yes, Glut1 Antibody (A-4): sc-377228 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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