Anti-GluR-1 Antibody (G-12) recommended for detection of GluR-1 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA

Anti-GluR-1 Antibody (G-12): sc-55509

Anti-GluR-1 Antibody (G-12) is rated 5.0 out of 5 by 12.
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Datasheets

    • Anti-GluR-1 Antibody (G-12) is a mouse monoclonal IgG1 κ GluR-1 antibody, cited in 30 publications, provided at 200 µg/ml
    • raised against a peptide mapping near the N-terminus of GluR-1 of human origin
    • GluR-1 Antibody (G-12) is recommended for detection of GluR-1 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and avian
    • GluR-1 Antibody (G-12) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GluR-1 (G-12): sc-55509.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GluR-1 Antibody (G-12) for WB and IHC(P) applications. These reagents are now offered in bundles with GluR-1 Antibody (G-12) (see ordering information below).
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I would like to find a GRIA1 antibody that works in zebra finch. The data sheet indicates that sc-55509 is suitable for avian. What is that based on?

Asked by: crolson
Thank you for your question. This is based on sequence analysis against chicken (Gallus gallus).
Answered by: Technical Support
Date published: 2019-04-13

I would like to use GluR-1 (G-12): sc-55509 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-55509 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-24
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Rated 5 out of 5 by from high quality It is very good. We used about 40ug protein during WB. The concentration of primary antibody is 1:1000 and concerntration of secondary is 1:5000. Exposure time is about 15s-30s. And we can see the band between100 and 120kd.
Date published: 2018-03-09
Rated 5 out of 5 by from works great f This antibody works great for GluR-1 protein expression detection, using a 1:500 dilution.Specific band at 106kda.
Date published: 2017-09-02
Rated 5 out of 5 by from Good antibody works good with 1:500 dilution. Signal is OK, clean and moderate. Maybe higher concentration will give better result.
Date published: 2017-07-27
Rated 5 out of 5 by from western blot Very nice western blot results; strong signal, low background.
Date published: 2017-06-03
Rated 5 out of 5 by from strong signal This is very popular detect GluR-1 for WB analysis, the signal is always strong.
Date published: 2017-05-27
Rated 5 out of 5 by from this antibody is easy to use. I got the GluR-1(G-12) mouse antibody two weeks ago. The mouse hippocampus tissue extracted protein, can be exposed with a band of about 105 kD, extraction of protein from N2a cells did not got the band, with a dilution of 1: 200.
Date published: 2017-03-27
Rated 5 out of 5 by from Good antibody ,, Very good antibody, used in WB on mouse rain at dilution 1/200.
Date published: 2017-02-12
Rated 5 out of 5 by from Excellent GluR-1 antibody for WB This mono can be used to detect both phosphorylated and nonphosphorylated GluR-1 in WB.
Date published: 2016-12-23
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