Glucagon Antibody (4j80)recommended for detection of Glucagon of mouse, rat, human, porcine, feline and canine origin by WB, IP, IF and IHC(P)

Glucagon Antibody (4j80): sc-71152

Glucagon Antibody (4j80) is rated 4.0 out of 5 by 2.
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Datasheets

    • Glucagon Antibody (4j80) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against polymerized Glucagon of porcine origin
    • recommended for detection of Glucagon of mouse, rat, human, porcine, feline and canine origin by WB, IP, IF and IHC(P); not recommended for detection of enteroglucagon
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Glucagon (C-11): sc-514592 for Glucagon antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can Glucagon (4j80): sc-71152 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, Glucagon (4j80): sc-71152 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Pretty well anti-Glucagon ab I recently purchased this antibody which stained alpha cells in mouse islet paraffin fixed-slides pretty well.
Date published: 2017-09-27
Rated 3 out of 5 by from Published immunoperoxidase staining using Published immunoperoxidase staining using this antibody with murine pancreata sections. -SCBT Publication Review
Date published: 2013-06-05
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