GLP-1 Antibody (033-04)recommended for detection of GLP-1(7-37) and GLP-1(7-36) amide of human origin by ELISA

GLP-1 Antibody (033-04): sc-65388

GLP-1 Antibody (033-04) is rated 4.5 out of 5 by 4.
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Datasheets

    • GLP-1 Antibody (033-04) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against the N-terminus of GLP-1 of human origin
    • recommended for detection of GLP-1(7-37) and GLP-1(7-36) amide of human origin by ELISA
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What is the recommended blocking solution for GLP-1 (033-04): sc-65388 monoclonal antibody for ELISA?

Asked by: jenni
We reommend blocking for 1-2 hours at room temperature, or overnight at 4° C with PBS containing 1% BSA and 0.02% azide to block non-specific protein binding. Please find our full ELISA protocol here: https://www.scbt.com/scbt/resources/protocols/elisa-assays
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Try different experiment conditions low MW target band at 19kda,a little difficult to detect, I have adjust different conditions.I suggest use low voltage and long time transfer condition.
Date published: 2017-06-25
Rated 5 out of 5 by from Great antibody Used in WB(1/500) and got correct band,is very good
Date published: 2017-05-09
Rated 5 out of 5 by from weston blot We tested it by weston blot . Its band is clear for WB detection by dilution 1:300.
Date published: 2017-02-25
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