Gl Syn Antibody (E-4)recommended for detection of GI Syn of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Gl Syn Antibody (E-4): sc-74430

Gl Syn Antibody (E-4) is rated 4.6 out of 5 by 19.
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Datasheets

    • Gl Syn Antibody (E-4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-373 representing full length Gl Syn of human origin
    • Gl Syn Antibody (E-4) is recommended for detection of GI Syn of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Gl Syn Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Gl Syn (E-4): sc-74430.
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I am using Gl Syn (E-4): sc-74430 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from prodotto funzionante Inizialmente avevo ricevuto il prodotto vuoto, ma l'azienda è stata molto gentile e disponibile, inviandomi nuovamente il prodott. Ho provato questo anticorpo in immunofluorescenza, su fettine di midollo spinale di topo criostatate. Diluizione 1:200 e funziona bene, il segnale è pulito e specifico. Prossimamente lo proverò anche in WB
Date published: 2019-09-18
Rated 4 out of 5 by from Intermediate Antibody In WB Weak but clearly visible in WB of HCT11-derived EVs(Dilution 1:100 in 5% Milk/TBST)
Date published: 2019-09-04
Rated 1 out of 5 by from IF did not work Tried this antibody on cryosections (10µm) from human retinal organoids (1:200/1:100/1:50) and did not get any signal.
Date published: 2019-07-23
Rated 5 out of 5 by from Very Efficient We tried this antibody in a rat cell line with 1:200 was diluted to WB, the band was strong, clear and successful.
Date published: 2017-11-02
Rated 5 out of 5 by from Effect is Quite Good Gl Syn Antibody prove helpful and efficient, it shows hight express in rat spleen tissue.
Date published: 2017-10-24
Rated 5 out of 5 by from Nice antibody Knowledge of the sensitivity and specificity of Gl Syn Antibody leads to their more rational selection.It worked really well.
Date published: 2017-10-18
Rated 5 out of 5 by from Strong and specific Clear, strong signal and low background when used for IHC on paraffin sections of mouse liver.
Date published: 2017-09-05
Rated 4 out of 5 by from JUST OK I applied it 2 weeks ago and have tried it in Westernblotting. I found specificity was just soso.
Date published: 2017-08-31
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