Giemsa Stain CAS: 51811-82-6
MF: C14H14ClN3S
MW: 291.8
A high quality stain for chromatin and the nuclear membrane.

Giemsa Stain (CAS 51811-82-6)

Giemsa Stain | CAS 51811-82-6 is rated 5.0 out of 5 by 1.
  • y_2021, m_7, d_27, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_203738, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 124ms
  • REVIEWS, PRODUCT
loading
5
1
4
0
3
0
2
0
1
0
Alternate Names: Giemsa solution
Application: Giemsa Stain is a high quality stain for chromatin and the nuclear membrane
CAS Number: 51811-82-6
Molecular Weight: 291.8
Molecular Formula: C14H14ClN3S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
loading

Giemsa Stain is a laboratory reagent and stain for microscopy. The stain functions by rearranging chromatin and inducing G bands producing a high quality stain for chromatin and the nuclear membrane. Giemsa Stains have been used for the histopathologic detection of malaria and other parasites such as spirochete and protozoan. The stain has also been incorporated in differential staining between bacterial and human cells. The compound has been used in combination with May-Grunwald or Wright Stain (sc-203763) as a histological hematology stain. However, the May-Grunwald-Giemsa procedure produces a more intense coloration than Wright-Giemsa.


References

1. McKay, R.D. 1973. Chromosoma. 44: 1-14. PMID: 4130184
2. Damsgaard, T.E., et al. 1997. Arch. Dermatol. Res. 289: 256-260. PMID: 9164634
3. Shapiro, H.M. and Mandy, F. 2007. Cytometry A. 71: 643-645. PMID: 17712779
4. Barcia, J.J. 2007. Int. J. Surg. Pathol. 15: 292-296. PMID: 17652540

Usage :
Technique used to uniformly stain chromosomes that will leave centromeres constricted:



Method 1

materials:

4 mL Giemsa; 96 mL pH 6.8 phosphate buffer



procedure:

1.Place slides in a Coplin jar or staining dish.

2.Prepare the working stain and pour it over the slides.
Stain for 7 minutes.

3.Rinse slides in two changes of distilled water.

4.Air dry slides; mount them with a cover slip if desired. (If sequential banding procedures are to follow, coverslipping is not recommended.)



Method 2

materials:

1.5N HCl

2.Distilled water

3.pH 6.8 phosphate buffer

4. Giemsa stain: 2 mL stock Giemsa; 4 mL pH 6.8 buffer; 92 mL distilled water



procedure:

1.Place slides in a Coplin jar containing 5N HCl for 10 minutes at room temperature.

2.Rinse with tap water for 10 minutes.

3.Stain for in Giemsa for 10 minutes.

4.Rinse, dry, and coverslip, if desired.
Physical State :
Solid
Solubility :
insoluble in water (<0.1%), methanol (5.8 mg/l), and ethanol.
Storage :
Store at room temperature
Melting Point :
300° C (lit.)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
MDL Number :
MFCD00081642
EC Number :
257-438-2
SMILES :
C[N+](=C1C=CC2=NC3=C(C=C(C=C3)N)SC2=C1)C.[Cl-]

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

Giemsa Stain (CAS 51811-82-6)  Product Citations

See how others have used Giemsa Stain (CAS 51811-82-6). Click on the entry to view the PubMed entry .

Citations 1 to 2 of 2 total

PMID: # 33080837  Int J Mol Sci.

PMID: # 25846425  Mol Med Rep. 12: 1653-8.

Citations 1 to 2 of 2 total
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 131ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Rated 5 out of 5 by from Barcia et al Barcia et al. (PubMed ID 17652540) discusses the Giemsa stain s diagnostic applications in malaria s plasmodium detection and chromatin staining. -SCBT Publication Review
Date published: 2015-03-25
  • y_2021, m_7, d_27, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_203738, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT