Anti-GFP Antibody (F56-6A1) recommended for detection of GFP by WB, IP and ELISA

Anti-GFP Antibody (F56-6A1): sc-53882

Anti-GFP Antibody (F56-6A1) is rated 5.0 out of 5 by 5.
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Datasheets

    • GFP Antibody (F56-6A1) is a mouse monoclonal IgG2b κ, cited in 22 publications, provided at 200 µg/ml
    • raised against full length GFP
    • recommended for detection of GFP by WB, IP and ELISA
    • See GFP (B-2): sc-9996 for GFP antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GFP Antibody (F56-6A1) for WB applications. These reagents are now offered in bundles with GFP Antibody (F56-6A1) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-53882: GFP (F56-6A1) monoclonal antibody?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Work well! I used this antibody in ChIP and It worked very well. I got a good result.
Date published: 2016-12-31
Rated 5 out of 5 by from This antibody worked well for staining our This antibody worked well for staining our pfa fixed human cells.
Date published: 2015-03-02
Rated 5 out of 5 by from Great monoclonal antibody Great monoclonal antibody, works well to specifically detect GFP.
Date published: 2014-11-04
Rated 5 out of 5 by from Published using this antibody in ChIP Published using this antibody in ChIP. Worked great! Also worked well for WB and IF.
Date published: 2014-01-21
Rated 5 out of 5 by from Good antibody for WB and IF Good antibody for WB and IF. We'd buy it again.
Date published: 2013-04-18
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