Anti-GFP Antibody (C-2) recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF and ELISA

Anti-GFP Antibody (C-2): sc-390394

Anti-GFP Antibody (C-2) is rated 4.1 out of 5 by 10.
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Datasheets

    • GFP Antibody (C-2) is a mouse monoclonal IgG2b κ, cited in 33 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 167-182 within an internal region of GFP of Aequorea victoria origin
    • GFP Antibody (C-2) is recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF and ELISA
    • Anti-GFP Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GFP (C-2): sc-390394.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for GFP Antibody (C-2) for WB applications. This reagent is now offered in a bundle with GFP Antibody (C-2) (see ordering information below).

We received a new lot of this antibody and it is not working (whereas a previous lot worked fine). Is it possible to get a replacement lot for free?

Asked by: Sammi
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-02-23

What secondary is best to use with GFP Antibody (C-2): sc-390394?

Asked by: AbPolly
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with our GFP Antibody (C-2): sc-390394, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 5 out of 5 by from GFP antibody This antibody works great for western blotting using traditional approaches.
Date published: 2020-07-27
Rated 4 out of 5 by from Good in western blotting in overexpressing tagged Dilution 1:1000 Western blotting in CFBE lysate celle overexpressing a protein wity an YFP tag Good signal at the expected molecular weight
Date published: 2019-12-11
Rated 1 out of 5 by from Did not work for IHC Tested on mouse tissue..... did not work for IHC. I simultaneously tested sc-9996 and sc-81045, both gave excellent signal for the same sample. For IHC application, I would recommend these two antibodies.
Date published: 2018-06-13
Rated 4 out of 5 by from Works for staining but also stains tdtomato The antibody works well for IF staining but also detects tdtomato so you may detect other RFP variants.
Date published: 2018-05-18
Rated 4 out of 5 by from Worked well in the detection of histone -egfp The antibody works best in immuno. However it required optimisation to detect a single specific band for fusion protein .
Date published: 2018-04-09
Rated 5 out of 5 by from good GFP detection Antibody produces clean results. It has been published for multiple applications and is a reliable monoclonal antibody.
Date published: 2017-01-13
Rated 5 out of 5 by from Excellent antibody! It's an excellent antibody that I got a good result when I used this antibody for WB detection of GFP fusion protein
Date published: 2016-12-31
Rated 5 out of 5 by from React well with transgenic plant protein sample. It is a free trial sample. It works better than the one I purchased anti-GFP.
Date published: 2016-12-13
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