Anti-GFP Antibody (B-2) recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA

Anti-GFP Antibody (B-2): sc-9996

Anti-GFP Antibody (B-2) is rated 4.8 out of 5 by 147.
  • y_2021, m_9, d_18, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_146
  • loc_en_US, sid_9996, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 7ms
  • REVIEWS, PRODUCT
loading
5
120
4
23
3
2
2
1
1
1

Datasheets

    • Anti-GFP Antibody (B-2) is a mouse monoclonal IgG2a κ GFP antibody, cited in 2,834 publications, provided at 200 µg/ml
    • raised against amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin
    • GFP Antibody (B-2) is recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA
    • Anti-GFP Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GFP (B-2): sc-9996.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for GFP Antibody (B-2) for WB applications. These reagents are now offered in bundles with GFP Antibody (B-2) (see ordering information below).
loading

Hi there, could you tell me if anti-GFP(sc-9996) antibody can detect eGFP protein by western blot?

Asked by: Kaipo
Thanks for your question.There's a 98% match between GFP and EGFP,so yes,it will work for eGFP.
Answered by: Technical Support 9
Date published: 2021-09-08

Has anyone used this GFP antibody for Aspergillus fumigatus??

Asked by: RorS
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Has this anti-GFP shown cross-reactivity with mClover? I've heard that anti-GFPs can cross-react with GFP derivatives (e.g. EGFP, YFP, mVenus, CFP, BFP etc.)

Asked by: Riordan
Please contact Technical Service
Answered by: Tech Sales
Date published: 2021-06-25

Does the following antibody work on frozen tissue sections?

Asked by: Fran9
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-08-01

Can this antibody detect CFP? 

Asked by: Vakil
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2021-01-06

Can this GFP antibody also be used to detect YFP in paraffin embedded tissues?

Asked by: Shaohai
Yes, this has been known to be reactive with YFP. http://onlinelibrary.wiley.com/doi/10.1002/hep.21458/full / PMID: 17326144
Answered by: Tech Service 1
Date published: 2020-12-09

I bought this antibody and it did not work for WB in rat trophoblast cells. I have two unspecific bands even in the control sample that has no GFP. What is your recommendation? I blocked the membrane with 5% milk in TBST and used concentrations of 1/200

Asked by: Ayelen
Thank you for your question. Please contact our technical service department directly for assistance regarding your WB results, we are happy to help troubleshoot this with you.
Answered by: Technical Service 3
Date published: 2020-11-20

What is the molecular weight that will show up on a western blot?

Asked by: jordanhollemb
Thank you for your question. The observed molecular weight of GFP is 27 kDa.
Answered by: Technical Support Europe
Date published: 2020-10-08
  • y_2021, m_9, d_18, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_42
  • loc_en_US, sid_9996, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 7ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Fantastic antibody This anti-GFP antibody always works nicely for us in Western blot detection of GFP-tagged plasmids transfected cell lysates from human lung endothelial cells. We have been using at 1:100 dilution. We also use this antibody for Immunoprecipitation which gives good consistent results.
Date published: 2021-09-07
Rated 5 out of 5 by from Good antibody A reliable GFP antibody for WB with a consistent performance.
Date published: 2021-06-25
Rated 4 out of 5 by from Worked well for brain IHC Worked well for human brain Immunohistochemistry at a very reasonable dilution.
Date published: 2021-04-28
Rated 3 out of 5 by from TFPには使えない? TFP発現マウスの脳切片を用いて様々な抗体濃度で免疫組織化学検出を試みましたがうまくいきませんでした。
Date published: 2021-04-21
Rated 5 out of 5 by from excellent for IP and WB Works beautifully for IP and WB using standard lysis buffers and 1:20000 goat-anti mouse HRP for detection (old developer machine).
Date published: 2020-12-09
Rated 5 out of 5 by from Excellent GFP ab A reliable GFP antibody for both WB and IP with a consistent performance.
Date published: 2020-11-17
Rated 5 out of 5 by from Great for IF We performed IF staining of the epithelial cells that overexpress GFP
Date published: 2020-09-17
Rated 5 out of 5 by from Great 293T細胞にGFP融合タンパク質を発現させてWestern blotを行いました。綺麗に検出することができました。
Date published: 2020-08-24
  • y_2021, m_9, d_18, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_146
  • loc_en_US, sid_9996, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT