Anti-GFAP Antibody (F-7) recommended for detection of GFAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-GFAP Antibody (F-7): sc-166458

Anti-GFAP Antibody (F-7) is rated 5.0 out of 5 by 5.
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Datasheets

    • GFAP Antibody (F-7) is a mouse monoclonal IgG1 κ, cited in 18 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-19 at the N-terminus of GFAP of human origin
    • Anti-GFAP Antibody (F-7) is recommended for detection of GFAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-GFAP Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GFAP (F-7): sc-166458.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GFAP Antibody (F-7) for WB and IHC(P) applications. These reagents are now offered in bundles with GFAP Antibody (F-7) (see ordering information below).
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I am using GFAP Antibody (F-7): sc-166458 for Western blot. How do I know if the band I am detecting is specific?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the blocking peptide sc-166458 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 5
Date published: 2017-01-24
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Rated 5 out of 5 by from Excellent choice for IHC and WB GFAP Antibody (F-7) is a very good antibody for IHC(P) and WB. Also very popular choice for IF as indicated in the numerous recent product citations.
Date published: 2017-01-07
Rated 5 out of 5 by from Nice immunofluorescence staining on rat striatum Nice immunofluorescence staining on rat striatum sections. -SCBT Publication Review
Date published: 2015-04-18
Rated 5 out of 5 by from Worked well for WB using synaptoneurosomes Worked well for WB using synaptoneurosomes. -SCBT Publication Review
Date published: 2015-02-22
Rated 5 out of 5 by from Published using F Published using F-7 for IF on rat glial extract. -SCBT Publication Review
Date published: 2015-01-03
Rated 5 out of 5 by from Strong clean band in human GFAP transfected Strong clean band in human GFAP transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-11-18
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