Geneticin (G418) SulfateAn aminoglycoside antibiotic

Geneticin (G418) Sulfate (CAS 108321-42-2)

Geneticin (G418) Sulfate | CAS 108321-42-2 is rated 4.8 out of 5 by 4.
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Synonym: Antibiotic G418
Application: An aminoglycoside antibiotic
CAS Number: 108321-42-2
Molecular Weight: 692.71
Molecular Formula: C20H40N4O102H2SO4
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Geneticin (G418) Sulfate is an aminoglycoside antibiotic that has originally been derived from Micromonospora rhodoranga NRRL 5326. This compound has been shown to cause a bypass of nonsense mutations during translation. Experiments have shown that geneticin sulfate inhibits the incorporation of amino acids into protein and can destroy fibroblasts. Mechanistic studies suggest that this agent affects the 80S ribosome and inhibits the elongation cycle. Further studies report that geneticin sulfate is highly active against amoebae, protozoa, tapeworm, and pinworm exposed murine cells.


References

1. Wagman, G.H. et al. 1974. Antimicrob. Agents Chemother. 6(2): 144-149. PMID: 15828184

2. Bar-Nun, S. et al. 1983. Biochim. Biophys. Acta. 741(1): 123-127. PMID: 6193810

3. Halaban, R. and Alfano, F.D. 1984. In Vitro. 20(5): 447-450. PMID: 6724622

4. Yang, C. et al. 2007. Proc. Natl. Acad. Sci. U.S.A. 104(39): 15394-15399. PMID: 17881586

Usage :
Potency is ≥700 μg/mg
Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (50 mg/ml) and aqueous buffers.
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
16760463
MDL Number :
MFCD00058314
SMILES :
CC(C1C(C(C(C(O1)OC2C(CC(C(C2O)OC3C(C(C(CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O

Download SDS (MSDS)

Certificate of Analysis

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Geneticin (G418) Sulfate  Product Citations

See how others have used Geneticin (G418) Sulfate. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 78 total

PMID: # 31211995  Brickner, DG.|Randise-Hinchliff, C.|Lebrun Corbin, M.|Liang, JM.|Kim, S.|Sump, B.|D'Urso, A.|Kim, SH.|Satomura, A.|Schmit, H.|Coukos, R.|Hwang, S.|Watson, R.|Brickner, JH.| et al. 2019. Dev. Cell. 49: 936-947.e4.

PMID: # 30606258  Guo, T.|Dakkak, D.|Rodriguez-Martin, T.|Noble, W.|Hanger, DP.| et al. 2019. Acta Neuropathol Commun. 7: 2.

PMID: # 30391349  Asahi, Y.|Nomura, F.|Abe, Y.|Doi, M.|Sakakura, T.|Takasuna, K.|Yasuda, K.| et al. 2019. Eur. J. Pharmacol. 842: 221-230.

PMID: # 30972741  Koske, I.|Rössler, A.|Pipperger, L.|Petersson, M.|Barnstorf, I.|Kimpel, J.|Tripp, CH.|Stoitzner, P.|Bánki, Z.|von Laer, D.| et al. 2019. Int. J. Cancer. 145: 1958-1969.

PMID: # 30718488  Arimura, Y.|Tachiwana, H.|Takagi, H.|Hori, T.|Kimura, H.|Fukagawa, T.|Kurumizaka, H.| et al. 2019. Nat Commun. 10: 576.

PMID: # 30718920  Li, Z. et al. 2019. Oncogene.

PMID: # 30110365  Hinman, A.|Holst, CR.|Latham, JC.|Bruegger, JJ.|Ulas, G.|McCusker, KP.|Amagata, A.|Davis, D.|Hoff, KG.|Kahn-Kirby, AH.|Kim, V.|Kosaka, Y.|Lee, E.|Malone, SA.|Mei, JJ.|Richards, SJ.|Rivera, V.|Miller, G.|Trimmer, JK.|Shrader, WD.| et al. 2018. PLoS ONE. 13: e0201369.

PMID: # 29571767  Conde, MA. et al. 2018. Biochim. Biophys. Acta. 1863: 639-650.

PMID: # 30038373  Wang, J.|Zhou, Y.|Fei, X.|Chen, X.|Chen, Y.| et al. 2018. Sci Rep. 8: 11094.

PMID: # 28937686  Castrogiovanni, C. et al. 2018. Cell Death Differ. 25: 190-203.

Citations 1 to 10 of 78 total

What is the purity of this product?

Asked by: Sanwid
Thank you for your question. Each lot of this chemical comes with a lot-specific Certificate Of Analysis which typically describes the percent purity as well as the method used to determine the purity. You may request a lot-specific Certificate Of Analysis on our website. Alternatively, please contact our Technical Service Department to inquire about the purity of our most recent lot.
Answered by: Technical Support
Date published: 2019-01-26

What is the potency of this chemical?

Asked by: 13punMA
Thank you for your question. Geneticin (G418) Sulfate Potency has been determined for previous lots to be ≥700 μg/mg. You may request a lot-specific CoA on our website for current information.
Answered by: Technical Support
Date published: 2017-03-09

Is this cell culture grade? Have the endotoxin levels been tested? And is sterility guaranteed?

Asked by: 13punMA
This product has not been specifically tested for cell culture use and is not cell culture grade, however, most products can still be used in cell culture applications by following basic cell culture protocols, e.g. using cell culture grade/sterilized solvents, aseptic technique, and sterilely filtering in a sterile hood. The endotoxin levels are not normally tested but a custom assessment can be done for an additional fee. Sterility cannot be guaranteed.
Answered by: Tech Service 11
Date published: 2018-02-12

After dissolving Geneticin (G418) Sulfate (CAS 108321-42-2) in water, where should I store it? Can it be frozen long term?

Asked by: two2igm05
We recommend that solutions be used the same day they were made. For short term storage (less than 6 months), the solution may be aliquotted it into useable amounts and stored frozen (-20ºC to -80ºC). Multiple freeze/thaw cycles should be avoided.
Answered by: Tech Service 11
Date published: 2017-02-24
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Rated 5 out of 5 by from works as promised Good price when buying in bulk; fast and efficient order processing
Date published: 2017-11-30
Rated 4 out of 5 by from G418 Great product.. it worked beautifully in U2OS cells
Date published: 2017-02-02
Rated 5 out of 5 by from Used in cell selection Used in cell selection, worked great.
Date published: 2015-04-05
Rated 5 out of 5 by from By treating HeLa cells with 8 g/ml of Geneticin By treating HeLa cells with 8 g/ml of Geneticin (G418) Sulfate for 48 hours, one can observe great cell death by staining with trypan blue. This compound is found to inhibit the incorporation of amino acids into proteins. -SCBT QC
Date published: 2015-02-20
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