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GDF-9B Antibody (F-7) | SCBT - Santa Cruz Biotechnology
GDF-9B Antibody (F-7) recommended for detection of mature and precursor GDF-9B of human and hamster origin by WB, IP, IF, IHC(P) and ELISA

GDF-9B Antibody (F-7): sc-271824

GDF-9B Antibody (F-7) is rated 5.0 out of 5 by 2.
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Datasheets

    • GDF-9B Antibody (F-7) is a mouse monoclonal IgG1 κ GDF-9B antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 268-350 mapping near the C-terminus of GDF-9B of human origin
    • GDF-9B Antibody (F-7) is recommended for detection of mature and precursor GDF-9B of human and hamster origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-GDF-9B Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GDF-9B (F-7): sc-271824.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GDF-9B Antibody (F-7) for WB and IHC(P) applications. These reagents are now offered in bundles with GDF-9B Antibody (F-7) (see ordering information below).
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We have done the experiment in cat ovary lysate in RIPA buffer and found that there are non-specific bands on the membrane. We would like to know whether this antibody works well and specific with BMP-15 in cat tissue or not? Do you have any suggestion?

Asked by: PROMPORN
Thank you for your question. Anti-GDF-9B Antibody (F-7) is only recommended for detection of mature and precursor GDF-9B of human and hamster origin by WB, IP, IF, IHC(P) and ELISA. It has not been tested in feline cell or tissue samples and is not recommended for such uses.
Answered by: Tech Support Europe
Date published: 2022-10-18

请问,这个牛上能做吗 GDF9B

Asked by: GDF9B
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请将您的目的蛋白序列发送到我们的技术支持部门。我们将对比您的序列以及我们的数据,以解答您物种反应性的相关问题。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-10-08

I am using GDF-9B (F-7): sc-271824 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Robust signal in WB in HL Robust signal in WB in HL-60 whole cell lysate. -SCBT QC
Date published: 2015-05-11
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic staining Great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human ovary tissue. -SCBT QC
Date published: 2015-03-19
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