Anti-GDF-15 Antibody (H-2) recommended for detection of precursor and mature GDF-15 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-GDF-15 Antibody (H-2): sc-515675

Anti-GDF-15 Antibody (H-2) is rated 4.0 out of 5 by 1.
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Datasheets

    • GDF-15 Antibody (H-2) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 41-62 near the N-terminus of GDF-15 of rat origin
    • Anti-GDF-15 Antibody (H-2) is recommended for detection of precursor and mature GDF-15 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-GDF-15 Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GDF-15 (H-2): sc-515675.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for GDF-15 Antibody (H-2) for WB applications. These reagents are now offered in bundles with GDF-15 Antibody (H-2) (see ordering information below).
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Hi, could you please tell me what the enterotoxin levels are for this product?

Asked by: Giuseppe
Thank you for your question. Enterotoxin levels are not determined for this product.
Answered by: Tech Support Europe
Date published: 2022-08-17

Please tell me the blocking peptide corresponding to this antibody (sc-515675).

Asked by: yuki13
Thank you for your question. We do not offer a blocking peptide for this antibody.
Answered by: Tech Support Europe
Date published: 2021-02-04

I have a question. is western blot data non-reducing conditions or reducing conditions? I did WB to detect gdf15 in LNCaP by non-reducing conditions. I got 2 band : one above 40 kDa, the other around 35 kDa.

Asked by: Col12
Thank you for your question. Our Western blot data with this antibody is under reducing conditions. We have not tested the antibody under non-reducing conditions.
Answered by: Technical Support
Date published: 2019-05-25

I have a rat cell line, can this ab detect GDF15 from rat?

Asked by: M76gmm
Thank you for your question. Yes, this antibody is recommended for detection of GDF15 of mouse, rat and human origin. We have Western blot data on our website in the rat cell line, RIN-m5F.
Answered by: Technical Support
Date published: 2019-01-31

Has anyone any information that this Ab can detect human GDF15. According to the protein alignments in aa 41-62 the Human sequence differs by an additional 7 amino acids. Would be keen to see some experimental data if anyone has it. Thanks

Asked by: Satish Patel
Thank you for your question. Yes, we have human cell data on this antibody in LNCaP cells on the product page: https://www.scbt.com/scbt/product/gdf-15-antibody-h-2?requestFrom=search If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-28

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-515675: GDF-15 (H-2) mouse monoclonal antibody will work?

Asked by: Dr Ninau Qelp
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 4 out of 5 by from Works well on murine cardiac samples 1h blocking in mPBST, GDF15 ab ON at 4°C, secondary ab 1 h at RT; Ab detected 2 bands: one slightly above 40 kDa, the other around 25 kDa
Date published: 2017-11-27
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