Anti-GATA-5 Antibody (D-5) recommended for detection of GATA-5 of mouse and rat origin by WB, IP, IF and ELISA

Anti-GATA-5 Antibody (D-5): sc-373684

Anti-GATA-5 Antibody (D-5) is rated 3.0 out of 5 by 2.
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Datasheets

    • Anti-GATA-5 Antibody (D-5) is a mouse monoclonal IgG2a κ GATA-5 antibody, cited in 1 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 369-397 at the C-terminus of GATA-5 of mouse origin
    • Anti-GATA-5 Antibody (D-5) is recommended for detection of GATA-5 of mouse and rat origin by WB, IP, IF and ELISA
    • Anti-GATA-5 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-373684 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GATA-5 (D-5): sc-373684.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for GATA-5 Antibody (D-5) for WB applications. These reagents are now offered in bundles with GATA-5 Antibody (D-5) (see ordering information below).
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I cannot make a normal band in Western blot, even many experiments were repeated and a series of GATA5 antibody concentrations such as 1:1000,1:500,1:200 were implemented.

Asked by: Dr min
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2020-07-08

how can i get antibodies free sample??

Asked by: SHISHIR
Thank you for your question. If you are in India, you may contact one of our authorized local distributors. Thank you. Your local distributors: https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support 8
Date published: 2017-12-25

What application is the blocking peptide sc-373684 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from How can i make a normal western band in this one I cannot make a normal western band by using this antibody, even i use the concentration in 1:1000,1:500 and 1:200. I just get nothing or a very vague band when the secondary antibody was 1:1000. I wish your replay.
Date published: 2020-06-19
Rated 5 out of 5 by from Good signal strength for western blot analysis Good signal strength for western blot analysis of mouse recombinant GATA-5 fusion protein. - SCBT QC
Date published: 2014-03-24
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